pH急性胁迫对许氏平鲉幼鱼生理及群体行为的影响
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1.大连海洋大学辽宁省海洋牧场工程技术研究中心, 辽宁 大连 116023 ; 2.大连海洋大学海洋牧场产业研究院, 辽宁 大连 116023 ; 3.设施渔业教育部重点实验室, 辽宁 大连 116023

作者简介:

王轲翔(1999-),男,硕士研究生,研究方向为水生生物环境胁迫.E-mail:1158532872@qq.com

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S917

基金项目:

国家重点研发计划项目(2023YFD2401103); 国家自然科学基金重点项目(42030408)


Effects of acute pH stress on physiology and group behavior of juve niles Sebastes schlegelii
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1.Center for Marine Ranch Engineering Science Research of Liaoning, Dalian Ocean University, Dalian 116023 , China ; 2.Industry Institute of Marine Ranching, Dalian Ocean Oniversity, Dalian 116023 , China ; 2.Key Laboratory of Environment Controlled Aquaculture of Ministry of Education, Dalian Ocean University, Dalian 116023 , China

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    摘要:

    为研究pH急性胁迫对许氏平鲉(Sebastes schlegelii)幼鱼生理及行为的影响, 以平均体重(19.80±1.36) g、平均体长(9.03±0.24) cm 的许氏平鲉幼鱼为研究对象, 设置6.5、7.5、8.5 (对照组)、9.5这 4个 pH梯度开展96 h室内模拟养殖实验, 研究许氏平鲉幼鱼在不同pH条件下的抗氧化性、免疫能力、肝脏损坏情况及群体行为反应。结果显示: (1) 与对照组相比, 许氏平鲉幼鱼各实验组抗氧化酶活性显著升高(P<0.05), 免疫酶和肝脏酶类活性随时间均有显著变化(P<0.05), 各实验组超氧化物歧化酶(SOD)活性在72 h较24 h显著升高(P<0.05), 免疫酶类活性呈现逐渐降低趋势。各实验组谷丙转氨酶(GPT)活性呈先降低后升高趋势, pH 6.5实验组中于24 h后显著降低(P<0.05), pH 7.5 实验组无显著变化(P>0.05)。pH 7.5、9.5 实验组谷草转氨酶(GOT)活性在72 h内呈现逐渐升高趋势, 在72 h 开始显著降低(P<0.05)。(2) 在 pH 6.5条件下, 许氏平鲉集群行为明显, 其个体间距离较对照组显著减小(P<0.05), 活跃性显著降低(P<0.05)。研究结果表明, 不同的pH会影响许氏平鲉幼鱼生理和行为。在pH 6.5和pH 9.5环境中, 许氏平鲉会降低活跃性来应对pH胁迫, 同时激活抗氧化系统、提高酸、碱性磷酸酶活性以增强免疫力从而主动调节体内生理平衡。

    Abstract:

    The purpose of this study was to examine how Sebastes schlegelii responded to acute pH stress in terms of group behavior and physiological variations. The pH of the water column is lowered during industrial aquaculture due to the high shellfish mortality, excessive algae cultivation, and seawater red tides. Water’s pH rises as a result of the massive consumption of carbon dioxide in the water caused by photosynthesis. Numerous enzymes, including those involved in immunology, antioxidant, and metabolism in aquatic organisms, are impacted by pH variations. Under stress, fish frequently run, reduce their metabolism, and increase the activity of related enzymes such as SOD and CAT. When fish are subjected to external stimuli, physiological and biochemical indices can represent their overall health ability to adapt to their environment. In this study, the effects of acute pH stress on the immunity, liver function, antioxidant activity, and group behavior of juvenile S. schlegelii were investigated. Different pH values (pH 6.5, pH 7.5, pH 8.5, and pH 9.5) in seawater were studied to identify the effects of pH stress on immunity, liver function, antioxidant activity, and group behavior of juvenile S. schlegelii, The results show that S. schlegelii CAT activity significantly increased in the pH 7.5 and pH 9.5 experimental groups at 48 h (P<0.05), SOD enzyme activity increased significantly (P<0.05) at 72 h compared to 24 h in all experimental groups and started to decrease after 72 h. ACP activity showed a gradual decrease in the pH 6.5 and pH 9.5 experimental groups, with a rebound in the pH 9.5 experimental group at 96 h but the change was not significant (P>0.05). At 48 h, AKP fell dramatically in the pH 6.5 and pH 9.5 experiment groups (P<0.05), but rebounded somewhat in the pH 6.5 group at 72 h (P>0.05). GPT decreased and then increased in all experimental groups with a significant drop in the pH 6.5 group after 24 h (P<0.05). There was no significant difference among the pH 7.5 experiment groups (P>0.05). Throughout the 48 h–72 h period, GOT activity gradually increased in the pH 7.5 and pH 9.5 experimental groups, although it significantly decreased at the start of the 72 h (P<0.05). In comparison to the control group, S. schlegelii’s degree of aggregation increased greatly and its activity dramatically decreased under pH 6.5 and pH 9.5 (P<0.05). The results showed that different pH levels influenced S.schlegelii’s physiological processes and behavioral decisions. The immunity of juvenile S.schlegelii was lower in the pH 6.5 and pH 9.5 experimental groups.

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王轲翔,田涛,岳跃,于晓明,吴忠鑫,李青霞. pH急性胁迫对许氏平鲉幼鱼生理及群体行为的影响[J].中国水产科学,2024,31(10):1163-1173
WANG Kexiang, TIAN Tao, YUE Yue, YU Xiaoming, WU Zhongxin, LI Qingxia. Effects of acute pH stress on physiology and group behavior of juve niles Sebastes schlegelii[J]. Journal of Fishery Sciences of China,2024,31(10):1163-1173

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  • 收稿日期:2024-07-22
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  • 在线发布日期: 2025-01-02
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