Out-season crab at lunar June (Eriocheir sinensis) refers to a juvenile male crab that has not completed reproductive molting, which occurs in June as per the lunar calendar. In recent years, the breeding scale and supply of the outseason crab at lunar June from the main river crab production area in China have increased rapidly. However, the edible yield and nutritional composition of the outseason crab from different areas are unknown. In this study, the edible yield, color parameters, proximate composition, and fatty acid composition of the outseason crabs at lunar June from six major crab producing areas in China (Huzhou in Zhejiang Province, Yangcheng Lake in Jiangsu Province, Chongming in Shanghai, Changdang Lake in Jiangsu Province, Hongze Lake in Jiangsu Province, and Weishan Lake in Shandong Province) were compared. The meat yield, hepatopancreas index, and total edibility of the outseason crab at lunar June ranged from 10.17%–11.87%, 27.83%–29.23%, and 39.44%– 40.37%, respectively, with no significant differences found among the groups (P＞0.05). The L* (brightness) and b* (yellowness) values of the outseason crab at lunar June from Weishan Lake were significantly higher than those of crabs from other regions (P＜0.05). Further, the outseason crab at lunar June from Yangcheng Lake had the highest a* (redness) values; crab hepatopancreas from Huzhou had the lowest L* and b* values; and outseason crabs from Weishan Lake had the highest L*, a*, and b* values. The hepatopancreas of the outseason crab at lunar June from Chongming had the highest moisture and ash. In addition, the crude protein and crude fat contents of the outseason crab at lunar June from Hongze Lake were significantly higher than those of the crabs from Huzhou (P＜0.05). The moisture, crude fat, and ash contents of the muscles of the six groups were not found to differ (P＞0.05) significantly. Moreover, only the crude protein contents of the outseason crab at lunar June from Hongze Lake and Weishan Lake were significantly higher than those of the crabs from Huzhou and Changdang Lake (P＜0.05). The total polyunsaturated fatty acid (∑PUFA) content in the hepatopancreas of the outseason crab at lunar June from Hongze Lake was significantly higher than that of the crabs from Yangcheng Lake and Weishan Lake. Further, these crabs had the highest DHA, EPA, and total long-chain polyunsaturated fatty acid (∑LC-PUFA) content (P＜0.05). The muscle of outseason crab at lunar June from Huzhou had the highest ∑PUFA content, while the muscle of the outseason crab at lunar June from Hongze Lake had the highest DHA content. The n-3/n-6 ratio of the crab from Hongze Lake was also significantly higher than that of the crab from Huzhou (P＜0.05). In summary, the edible yield and proximate composition of the outseason crab at lunar June from the six major production areas were similar; however, large differences in color and fatty acid composition were found among these crabs. The a* and b* values of the outseason crab at lunar June from Yangcheng Lake and Weishan Lake were relatively high. Further, the edible tissues of the outseason crab at lunar June from Yangcheng Lake, Changdang Lake, and Hongze Lake had high DHA content, which may be related to their breeding environment and feeding bait.