Detection of Nile tilapia specific antibody secreting cells induced by human IgG via ELISPOT assay

doi:10.12264/JFSC2022-0206

Home > Archive>Volume 29, Issue 10, 2022 >1417-1424. DOI:10.12264/JFSC2022-0206

Detection of Nile tilapia specific antibody secreting cells induced by human IgG via ELISPOT assay
DOI:
                        10.12264/JFSC2022-0206
                    
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                        HUANG YuHUANG Yu
Fisheries College, Guangdong Ocean University, Zhanjiang 524088 , China ；Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088 ,China ；Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions,Zhanjiang 524088 , China
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PANG BijianPANG Bijian
Fisheries College, Guangdong Ocean University, Zhanjiang 524088 , China ；Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088 ,China ；Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions,Zhanjiang 524088 , China ；Marine Environmental Monitoring Center of Guangxi, Beihai 536000 , China
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LI QiLI Qi
Fisheries College, Guangdong Ocean University, Zhanjiang 524088 , China ；Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088 ,China ；Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions,Zhanjiang 524088 , China
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WU ZaoheWU Zaohe
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088 ,China ；Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions,Zhanjiang 524088 , China
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JIAN JichangJIAN Jichang
Fisheries College, Guangdong Ocean University, Zhanjiang 524088 , China ；Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088 ,China ；Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions,Zhanjiang 524088 , China
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Abstract:

Specific antibody secreting cells (ASC) play an important role in the immune response. In this study, to detect changes in specific ASCs in the Nile tilapia (Oreochromis niloticus) in response to exogenous stimuli, the number of ASCs in the head kidney, peripheral blood, and spleen of this fish after immunization with human IgG was detected by enzyme-linked immunospot assay (ELISPOT). The results showed that ASCs could be detected in the head kidney on day 1 after the first immunization, while ASC could be detected in the peripheral blood and spleen on day 3. The number of ASCs in the head kidney, peripheral blood, and spleen all reached their peak on day 12. While the number of ASCs in the kidney and peripheral blood was significantly reduced, that in the spleen was not significantly reduced. Subsequently, ASCs could be detected in the head kidney, peripheral blood, and spleen on day 1 after the second immunization, with the number of ASCs reaching their peak on day 9, earlier than the first immunization. In both the first and second immunizations, the number of ASCs in the head kidney tissue was the highest among the three tissues. Using enzyme linked immunosorbent assay (ELISA) to detect the antibody levels in serum after the first and second immunizations, the change trend was found to be the same as that of the ASCs. These results indicate that the Nile tilapia developed immune memory after the first immunization and produced more ASCs and antibodies during the second immunization. Furthermore, the head kidney was identified as the main source of ASCs. These findings provide an important scientific basis for the study of immune memory in fish.

Key words:Nile tilapia; antibody secreting cell; ELISPOT; antibody; immune memory

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黄瑜,庞碧剑,李琦,吴灶和,简纪常. 人IgG诱导尼罗罗非鱼特异性抗体分泌细胞的ELISPOT检测[J]. Jounal of Fishery Sciences of China, 2022,[volume_no](10):1417-1424

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Online: October 31,2022
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