Analysis of feed conversion ratio, bodyweight, body thickness traits of common carp (Cyprinus carpio L.) using SSR, EST-SSR, SNP markers
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1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;2. College of Fisheries and Life Science , Shanghai Ocean University, Shanghai 201306, China;3. College of Aqua-life Science and Technology, Dali

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    Abstract:

    hybrid common carp gynogenetic line including 68 individuals was used as mapping pane1. Markers wNinety-one SSR markers and 33 EST makers and 364 SNP markers were selected to analyze the genomic DNA of 68 progenies derived from common carp full-sib family. Twenty-seven loci were discovered that had significant impact on feed conversion efficiency and thirty-five loci were significantly related with body thickness (<0.05)while twenty seven loci had significant impact on body weight (<0.05). SNP0041 and SNP0044 were related with feed conversion ratio significantly (<0.01), contributing to 15% and 16%, respectively. These markers can be used as molecular marker assisting breeding. Markers were used to screen the protein and nucleotide database in the National Center for Biotechnology Information (NCBI) in order to find the homologies. Blasting results showed that HLJ253 is highly correlated (61%) with the gene encoding structural protein (ORF2) of zebrafish. SNP makers associated with three traits were sequenced on NCBI in order to find related genes and possible functions.

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王宣朋,张晓峰,李文升,张天奇,孙效文. 利用SSR﹑EST-SSR、SNP标记对鲤食物转化率、体厚、体质量3种性状的分析[J]. Jounal of Fishery Sciences of China, 2011,[volume_no](3):565-573

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  • Online: May 09,2011
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