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Effects of activating mediums with different salinity, potassium ion and conservation time on sperm motility of Anguilla japonica
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1. Freshwater Fisheries Research Center, Chinese Acdemy of Fishery Sciences, Wuxi 214081, China;2. Key Laboratory of Freshwater Ecology and Aquaculture; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, Chin

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    Abstract:

    treated with six activation mediums with different salinities(34, 32, 30, 28, 26 and 22), three extender solution with different potassium ion concentration(25mmol/L,30 mmol/L,35mmol/L) and different conservation time(0h, 24h, 48h, 72h and 96h) was observed and measured. The results showed that: when activation medium salinity was 30, both sperm motility and percentage of motile sperms were the highest, meanwhile the curvilinear velocity(VCL), the straight line velocity(VSL) and the angular path velocity(VAP) were the highest. It could be concluded that the spawning salinity of may be 30 in natural environment. When potassium ion concentration in extender solution was 30mmol/L, both sperm motility and percentage of motile sperms were the highest, meanwhile the curvilinear velocity(VCL), the straight line velocity(VSL) and the angular path velocity(VAP) were markedly higher than those under other concentration levels(0.05. So the best osmotic pressure of extender solution may be 796.68kPa. As the conservation time extended, sperm motility reduced gradually. When potassium ion concentration in extender solution was 30mmol/L, conservation time of sperm could reach 96h and sperm survival rate was higher than in 25mmol/L and 35mmol/L potassium ion.

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张涛,柳凌,郭峰,张洁明. 不同盐度激活液、K+浓度及保存时间对日本鳗鲡精子活力的影响[J]. Jounal of Fishery Sciences of China, 2011,[volume_no](3):523-530

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  • Online: May 09,2011
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