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Preparation of recombinant immobilization antigen of Ichthyophthirius multifiliis 
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[cstr]
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1. Biotechnology Institute, Fujian Academy of Agricultural Sciences, 2. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences

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S942

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    Abstract:

    Themolecularweightoftherecombinantimmobilizationantigenis35.7kD.Inaddition,westernblottingcorroboratedthattherecombinantIaghadsimilarimmunogenicfeaturestotheIagofcentrifugation,ultrasonic,dialysisandbyusingQuil-Aastheadjuvant.ResultsfromnegativecontrastelectronmicroscopyobservationsindicatethatIag-ISCOMisacage-likestructurewithadiameterof30nm.TheIag-ISCOMsprovideprotectiontotheeelsbasedonthetrialsofimmunizationandchallenge.Thus,s.ResultsshowthattheIag-ISCOMswithimmunogenicityweresuccessfullyprepared.

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柯翎,陈如敬,刘晓东,杨金先,龚晖. 重组多子小瓜虫抑动抗原ISCOMs制备[J]. Jounal of Fishery Sciences of China, 2011,[volume_no](4):924-928

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  • Online: August 16,2011
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