The freshwater aquiculture industry has developed rapidly in China. As a result, China has become the largest producer of freshwater cultured fish in the world. , a widely distributed freshwater bacterial pathogen that is thought to cause disease in a range of aquatic animals. For example, is thought to cause bacterial septicemia in fish and red abdominal shell diseases in soft-shelled have resulted in significant economic losses to the aquaculture industry. In addition, is a zoonotic pathogen, sometimes causing gastroenteritis and septicemia in humans. Our objective was to document the epidemiological characteristics of pathogenic to provide insight into potential measures to prevent and control infections. We genotyped pathogenic strains collected from a variety of sources, determined their drug susceptibility, and evaluated the relationship between genotype and antimicrobial resistance patterns. We used collected and Guangdong.is a more rapid, efficient, andmolecular typing method than the traditional serotyping methods. In addition, we evaluated the susceptibility of each isolate to 12 antibacterial drugs and determined the relationship between the genotype and the antimicrobial resistance pattern. XII) from the 50 isolates. Among these, genotype VII was the dominant genotype, consisting of 14 strains, whereas genotype III and X contained only one strain. All strains were resistant to ampicillin and 98% of the strains were resistant to cefalexin. However, >94% of the strains were susceptible to aminoglycosides and quinolones. We identified 9 patterns of drug-resistance: AMP, CX/AMP, CX/AMP/POL, CX/AMP/SMZ, CX/AMP/NM, CX/AMP/SMZ/POL, CX/AMP/SMZ/POL/GM, CX/AMP/ SMZ/LOM/ENR, and CX/AMP/SMZ/LOM/ENR/OF/LEV. The twelve strain genotypes shared a common pattern of drug resistance (CX/AMP/POL). Similarly, the majority of genotypes within strain II and XI shared a common pattern ofdrugresistance (CX/AMP/SMZ). The drug-resistant pattern of most genotypes within strains IV, VI, and VIII was CX/AMP and for strain X the pattern was CX/AMP/NM. Thus, our results suggest that there is likely a correlation between the genotype and drug-resistance among strains.