We investigated the relationship between the eyed rate and the biochemical composition of eggs and ovarian fluid in , amino acids, vitamins, and minerals in stage V eggs and ovarian fluid. We used each of these parameters as an independent variable in a regression model to predict the eyed rateATPase activity, phosphorus levels, and amino acids in the eggs. There was no correlation between the eyed rate and the remaining parameters (protein, , succinate dehydrogenase, calcium ions, iron ions, vitamin C, and vitamin E). Egg batches with high viability (μmolPi/(min·mgprot), levels of phosphorus between 12.97 and 23.03 μmol/g egg, and levels of amino acids between 574.89 and 1 195.40 μmol/g egg. In contrast, in the ovarian fluid only Egg batches with high viability (spartate aminotransferase activity >18.14 μmol/min/L ovarian fluid.