SUN Jing1
1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China;2. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang 524025, China2
1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China;2. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang 524025, ChinaLI Guangli1
1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China;2. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang 524025, ChinaZHU Chunhua1
1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China;2. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang 524025, ChinaWU Tianli1
1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China;2. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang 524025, ChinaDENG Siping1
1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China;2. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang 524025, China1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China;2. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Guangdong Ocean University, Zhanjiang 524025, China
Clarius fuscus using RT-PCR and RACE. The cDNA was 2 347 bp with a 219 bp 5′UTR, a 596 bp 3′UTR (excluding poly (A)), and a 1 503 bp ORF, which encoded 500 amino acids and had a predicted mol wt of 56.388 kD. Sequence and phylogenetic analyses indicated that the Pelteobagrus fulvidraco, Carassius auratus, . In contrast, there was low sequence identity (<62%) with Cyp19a1b subfamily.This is consistent with the classification based on traditional morphology and biochemistry. mRNA was expressed primarily in the forebrain, hypothalamus, and pituitary, and to a lesser extent in the liver, testis and ovary. We observed differences in the level of expression in brain between males and females ( was expressed prior to sex differentiation in , but there was no difference in the level of expression between prior and post to sex differentiation (12cyp19a1b is not directly involved in mediating sex differentiation in , but may play an indirect role by acting on the hypothalamic-pituitary-gonadal axis.
孙晶,李广丽,朱春华,吴天利,邓思平. 胡子鲇脑型芳香化酶基因全长cDNA克隆及表达[J]. Jounal of Fishery Sciences of China, 2012,[volume_no](3):408-415
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