Isolation and identification of a flocculant-producing Bacillus sp. and analysis of the properties of the bioflocculant
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1. Ocean University of China, Qingdao 266071, China;2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

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S96

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    Abstract:

    maybeproducedbyvariouskindsofbacteria.Theyareusuallycomposedofglycoprotein,polysaccharide,celluloseandnucleicacid.AsMBFcanbeeasilybrokendownbymicroorganismsintheenvironment,theyhavepotentialapplicationinthesewagetreatmentindustry.playanimportantroleintheconversionoffoodenergyandinthemaintenanceof(hereinafterreferredtoas1102)fromrDNAsequenceanalysisandgaschromatographicwhole-cellfattyacidanalysiswereemployedtoidentifythebacterium1102.Toanalyzeitsflocculatingactivity,theMBFwasextractedandpurifiedfromthefermentationbrothofbacterium1102thathadbeenfermentedinGauze’smediumNo.1amyloliquefaciens1102was80.19%.ThehighestyieldofMBF)wasobtained72hafterinoculating.bygelpermeationchromatography,phenolsulfuricacidcolorimetry,andautomaticaminoacidanalysis,respectively.TheaveragemolecularweightofMBFwasabout7thepolysaccharidecontentwas58.58%,andtheaminoacidcontentwas2.49%.inmanycountriesandshowsagreatpotentialforinincreasingfoodconversionrateandforzerowaterexchange.BFTusuallydependonmicroorganismsthatareabletoproduceMBFintheculturesystem.Bacterium1102ishighlyefficientforproductionofMBFathighflocculationratesandcanbeusedinmarineaquaculturetoflocculateorganicdetritusandmicroalgaeinponds.Inaddition,bacterium1102isahelpfulbecausemanyspeciesofbacillusareprobioticforaquaculture.

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赵伟伟,王秀华,孙振,黄倢,朱岩松,史秀秀,付军,杨从海. 一株产絮凝剂芽孢杆菌的分离鉴定及絮凝剂特性分析[J]. Jounal of Fishery Sciences of China, 2012,[volume_no](4):647-653

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  • Received:
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  • Online: October 25,2012
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