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Full length cDNA cloning and tissue expression of the Na+-K+-ATPase α1 subunit from Cyprinus carpio var. jian
DOI:
CSTR:
[cstr]
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Affiliation:

1. College of Fisheries, Nanjing Agricultural University, Wuxi 214081, China; 2. Freshwater Fisheries Research Center, Key Laboratory of Freshwater Fisheries and Germplasm Resources

Clc Number:

Q959

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    Abstract:

    -ATPaseisaubiquitousplasmamembraneenzymethatandItsfunctioniscoupledtothehydrolysisofATPandNaplaysacentralroleincellioncontent,ofcells.S-subunitcontainsanintracellularATPbindingsite,aphosphorylationsite,andanextracellularbindingsiteforthemaintainingstabilityandcorrectmembraneorientation-subunithavebeenidentifiedinmammals.-subunitisexpressedubiquitouslyamongtissuesandisthoughttoplaya‘‘housekeeping’’roleinmaintainingNacellviability-ATPaseefull-lengthcDNAencoding+wasclonedfromusinghomologycloningandRACEPCR.The1subunitandthoseofother-ATPasehadhighsimilarity(91.97%Rhabdosargussarba,XenopuslaevisReal-timegenewastissues:brain,liver,kidney,intestines,spleen,andgills.-ATPasebrain,,andthe

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刘珊,李冰,张成锋,,魏可鹏,朱健. Na+-K+-ATPase α1基因全长cDNA的克隆及序列分析和表达[J]. Jounal of Fishery Sciences of China, 2012,[volume_no](4):594-603

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  • Online: October 25,2012
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