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Prokaryotic expression of growth hormone and its receptor genes in the tongue sole (Cynoglossus semilaevis) 
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Key Laboratory for Fishery Resources and Eco-environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

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Q959

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    Abstract:

    tonguesole()weresub-clonedintotheexpressionvectorspET-32aandpGEX-6P-1,respectively.Subsequently,thesesodiumdodecylsulfate-polyacrylamidegelelectrophoresis(SDS-PAGE).Asaresult,anewspecificGHbandexpressedbyusingthepET-32avector(pET-32a-CSGH)wasfoundwithmolecularmassofabout40kD,andanewspecificGHR-bandexpressedbyusingthepGEX-6P-1vector(pGEX-6P-1-CSGHR1-2)wasfoundwithmolecularmassofabout70kD.TheGHandGHR-recombinantproteinswereexpressedandcomposedof37.6%and20.2%ofthetotalbacterialproteins,respectively.However,pGEX-6P-1-CSGHandtheotherthreerecombinantplasmids(pET-32a-CSGHR1-1,pET-32a-CSGHR1-trans-membranedomainwerenotsuccessfullyexpressed.Therelevantresultsshowedthatthetrans-membranedomainoftheGHR-

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马骞,柳淑芳,庄志猛,唐启升. 半滑舌鳎生长激素及其受体基因的原核表达[J]. Jounal of Fishery Sciences of China, 2012,[volume_no](6):956-962

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  • Online: November 21,2012
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