arebecomingsignificantlyreduced.Fishcultureandartificialhatchingwillhavetoplayagreaterroleinprovidingfloundersources.GermplasmdegradationwithinfishcultureandtheeffectsofartificialhatchesonTherefore,analysisofthegeneticdiversitystatusofthewildfloundergroupattheDNAlevelisveryimportantforprotectionoffloundergermplasmresources.Inthisstudy,inordertoprovidethebasicdatatocorrectlyevaluatethefloundergermplasmresourcesandthegeneticdiversityofthewildgroup,18highlevelpolymorphismmicrosatellitemarkerswereusedtoscreenawildfloundergroupincluding90samples,capturedin2009attheQinhuangdaooffshorearea,HebeiProvince,China.Atotalof161allelesweredetected.Allelenumberinall18locirangedfrom70.86,withanaverageof0.80.ThemeanShannoninformationindexwasestimatedtobe1.9.Theminimumobservedheterozygositywas0.33andthemaximumwas0.64withthemeanbeing0.64.Theminimumexpectedheterozygositywas0.74,themaximumwas0.89andthemeanwas0.84.AtotalofninelociwereprovedsignificantlydeviatedfromtheHardy-WeinbergEquilibriumbytheχ<0.05).TheresultsindicatedthattheoffshoreflounderpopulationwithinQinhuangdaoisavaluablegermplasmresource,richingeneticinformation,andhadanevenalleledistributionandarelativelyhighlevelofgeneticdiversity.Asameasureofprotectingthenaturalgeneticdiversityoftheflounderpopulation,reducingtheimpactofreleasedgroupsonwildgroupsinaneffectivewayandimprovinggeneticdiversityofthefloundergroupintheopensea,brood-stocksinmosthatcheriesconsistedlargelyofculturedfloundersandasmallnumberofwildflounders.Therefore,periodicallychangingspawnbrood-stocksandincreasingthenumberofwildbrood-stockswillbethefirststeptoincreasethegeneticdiversityofreleasedfloundergroups,therebyreducingtheimpactofthehatchfloundersbeingreleasingtotheopenseaonwildfloundergroups.