Optimization of genomic DNA extraction from formalin-fixed fish specimens

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Optimization of genomic DNA extraction from formalin-fixed fish specimens 
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Affiliation:1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Clc Number:Q173
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Abstract:

Inrecentdecades,thepopulationstructureofmanymarinefishhasdramaticallychangedundertheinfluenceofanthropogenicactivitiesandclimatechange.Tobetterunderstandthechangemechanism,DNAextractedfromformalin-fixedmarinefishspecimensisrequired.However,obtaininghighqualityDNAfromformalin-fixedspecimensisdifficult.Toobtainknowledgeforspecimensthatwerefixedin1962wereusedassamples,andanorthogonalexperimentwasdesignedfollowingtheL)table.TheDNAconcentrationwastakenastheevaluationindex.Thetissuetypes,anti-formaldehydetreatments,digestivetimesandextractionmethodsweretakenasselectionfactors.Thebestgroupwasdeterminedtobe℃anddryinginavacuumcentrifuge,3hofdigestionwithProteinaseKwithinawaterbathof55,andDNAextractionbykit.Anti-formaldehydetreatmentssignificantlyimprovedtheproductionofDNAandtheuseofvariousanti-formaldehydemethodsobtainedthebesteffect.Digestionaftera90DNAwasassessedbyPCRamplificationandsequencingthemethods

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孔啸兰,陈作志,林琳,李纯厚,梁沛文. 福尔马林固定鱼类标本DNA提取方法的优化[J]. Jounal of Fishery Sciences of China, 2012,[volume_no](6):1068-1073

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Online: November 21,2012
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