Abstract:The food composition, spatial and trophic niche width, trophic-spatial niche overlap for five gobiid fishes in Jiaozhou Bay were studied using the Shannon-Wiener index and Pianka overlap index. The trophic-spatial niche overlap was calculated to describe the relationship between feeding competition and spatial distribution of these gobiid fishes. Results showed that all the five gobiid fishes could be classified as benthic carnivorous predators. fed mainly upon shrimps, polychaetes and fish. The main prey items ofTridentiger trigonocephalus and were some small benthic crustaceans such as copepods, amphipods and cumacea. Among the five gobiid fishes, T. trigonocephalus had the highest spatial niche width value (2.01). had the lowest values of trophic niche width (1.26) and spatial niche width (0.97). The trophic niche overlap values for five gobiid fishes ranged from 0.03 to 0.64, with having the highest trophic niche overlap value (0.64). The spatial niche overlap values were between 0.08 and 0.91, with having the highest spatial niche overlap value (0.91). The trophic-spatial niche overlap values ranged from 0.003 2 to 0.364 8, with having the highest trophic), which may result in intensive food competition. The competitions among other gobiid fishes were reduced by partitioning of trophic