Cytological study on fertilization process of Myxocyprinus asiaticus
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Key Laboratory of Aquatic Science of Chongqing, School of Life Science,Key Laboratory of Freshwater Fish Reproduction and Development, Education of Ministry, Southwest University, Chongqing 400715

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S965

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    Abstract:

    egg was studied using artificial fertilization, histology, ultrastructural technology methods etc. The observation showed that there is only one micropyle in shell of egg’s animal pole of belongs to the type of deep concave and short channel.At water temperature of 17sperm passed through micropylar apparatus at 3 s after insemination and entered ovum at 5 s. Sperm star light was formed at 10 s after insemination. After 15 min after insemination ovum was in late time of second meiotic division and second polar body appeared. Ovum was in telophase after 20 min and second meiotic division was formed. Male and female pronucleus was fused after 90 min. vum was in metaphase of first karyokinesis at 140 min after insemination. At 180 min after insemination first karyokinesis was over and second cell was formed. The results showed that in micropylar apparatus of mature egg there is no micropylar cell and other stuff, which is similar to that of most other teleost. The inner diameter of micorpylar apparatus is 2.42 µm and near to the diameter of sperm head. There are many spines in inner walls of anterior micropylar apparatus and holes are arranged in spiral way, which was one of the reasons for monospermy. This study aims to provide basic data for the research of artificial reproduction technology of .

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李萍,刘晓蕾,李勇,江星,金丽,张耀光. 胭脂鱼受精过程的细胞学观察[J]. Jounal of Fishery Sciences of China, 2013,[volume_no](2):250-259

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  • Online: April 03,2013
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