affinity chromatography, SDS-PAGE, western-blotting, antifungal assays, microscope and electron microscopic observation. activity at concentrations of 0.1–0.8 mg/mL. Furthermore, microscopic observation suggests that A. niger s. Electron microscopic analysis of indicated that the sporangium were atrophied, and the conidiospores were gathered into gouts. In addition, the hyphaes were cracked, contorted, and shortened, and the conidiosporangia were not visible. Taken together, our results suggest that hemocyanin possesses novel immune function (i.e., properties) likely related to inhibition of spore and hyphae growth.