mitochondrial COI gene of five cyprinoid aquaculture varieties were sequenced and their genetic diversity and cluster analysis were compared. Eight haplotypes were detected from 100 individuals,94.06 % belonged to five haplotypes (H1H4H6). Songpu mirror carp had five) was 0.160±0.070 ~ 0.811±0.055, the highest genetic distance between varieties (50.28% ranged from 0.711 4−0.831 2. Compared with the others varieties, exhibited the biggest differences. According to the evolutionary tree based on genetic distance, five varieties could be divided into three groups, the first group included Frigid carp and Red mirror carp, the second included Songpu mirror carp and Songpu carp, and the last included Cyprinus carpio , all varieties shared the same haplotypes (H2H5H6), which may be due to cross breeding. This study also indicated that DNA barcoding is a practical tool for analyzing the hereditary of different varieties. However, some limitations still exist