Adhesion to the host mucus, which is closely related to the virulence of pathogenic bacteria, is the first crucial step in most infections. In this paper, the adhesion characteristics of ) skin mucus was investigated by indirect ELISA. can be used as an index to reflect the number of adhering bacteria. Second, we confirmed that the iCFU. The number of a saturation kinetics equation =0.986) was obtained by plotting the number of adhering bacteria against cell density. The factors affecting bacterial adhesion were then investigated. The results revealed that the number of adhering increased with incubation time and reached a peak after 150 min. Temperature was one of the major factors affecting bacterial adhesion, with peak adhesion occurring at 20−28. pH also influenced bacterial adhesion. In a weak acid with a pH of 6.2−6.6, the adhesion ability of The optimum concentrations of Na⁺ and adhesion were 1.5−2.5 g/L and 3 g/L, respectively. In summary, the optimum conditions for the adhesion of to eel skin mucuswere 20‒28, and 150 min incubation time. In addition, we found that bacterial adhesion was influenced by carbohydrates. Seven kinds of carbohydrates were investigated and the results indicate that glucosehelp to further elucidate bacterial pathogenicity and help