Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
S941
By using cell culture and virus infection methods, a virus had been isolated from ) suffering with severe hemorrhage, which were cultured in a circulation aquaculture system in Wuhan,and then identified as Cyprinid herpesvirus 2(CyHV-2), designated as CyHV-2 WH strain, by electron microscopy observation, nested-PCR and viral DNA sequencing and sequence alignment analysis. The of diseased fish could cause typical cytopathic effect in Koi-Fin cells. In artificial infection test, the typical symptoms of hemorrhage could be reproduced as naturally infected fish by infecting both , and the death rate reached 100%. Electron microscopy observation of ultra-thin section samples of spleen and kidney tissues revealed that the viral was spherical in shape measuring 110–120 nm in diameter with a 170–200 nm envelope. The obtained by the nested-PCR assay of both tissue homogenate and cell culture. Sequence alignment analysis of the DNA fragment showed that WH (>99.44%) with the published DNA helicase gene sequence of CyHV-2 stains in GenBank, meanwhile, it shared 81.55% identity with CyHV-3 stains. Phylogenetic analysis showed that WH strain formed an independent branch on the phylogenetic tree with other CyHV-2 strains such as JS2012, H.Fukuda and YC110907.
徐进,曾令兵,杨德国,张辉,马杰,江南,范玉顶. 鲤疱疹病毒2型武汉株的分离与鉴定[J]. Jounal of Fishery Sciences of China, 2013,[volume_no](6):1303-1309
