Gene pyramiding is a method of obtaining good varieties, which aims to produce individuals with one superior economic trait, according to the optimal breeding scheme involving selection of favorable target alleles or linked markers after crossing basal populations and pyramiding them into a single individual. The largemouth bass () is one of the most important freshwater fish in China. Previous research identified certain molecular markers related to growth traits. In this study, eight molecular markers including four single nucleotide polymorphisms (SNPs) and four simple sequence repeats (SSRs) related to the growth traits of largemouth bass were selected to amplify the genomic DNA of parent largemouth bass. The four SNPs were located in the genes; the four SSRs were located in the genes. Twenty large mouth bass (9 females, 11 males) containing four or even more advantageous genotypes were selected as parents for population reproduction, which were selected from 196 individuals. About 5 000 offspring individuals were cultivated in one breeding pond (1 114 m² ) and fed with frozen fresh fish. After 9 months of cultivation, the offspring had reached a marketable size. Two hundred and eighty-eight individuals were randomly chosen to measure their weight and detect their genotype. The results showed that the number of advantageous genotypes in parents were 1–5 and the frequencies were 20.41%, 41.3%, 30.10%, 8.67% and 1.53% respectively. To aggregate the advantageous genotypes in the offspring, the parents that had 4 and 5 advantageous genotypes were selected. The number of advantageous genotypes was1–6 in all offspring and the average weights of 1 to 6 advantageous genotypes groups were 227.83, 239.56, 258.81, 273.02, 302.50 and 305.60 g, respectively. The number distributions of each population were 6, 100, 110, 44, 20 and 8. The frequencies of each population were 2.08%, 34.72%, 38.19%, 15.28%, 6.94% and 2.78%, respectively. A general linear model (GLM) was used to perform correlation analysis between the number of advantageous genotypes and growth traits. GLM analysis showed that the number of advantageous genotypes related to growth traits was positively correlated with weight. Individuals with five or six advantage genotypes were significantly different (<0.05) with those that had fewer advantageous genotypes. Further analysis suggested that the average number of advantageous genotypes is 2.99 in the offspring, which is truly improved compared with the average number of advantageous genotypes of the parent group (2.36). The results indicated that it was useful to improve the growth trait of largemouth bass using limited advantageous genotypes related to growth traits, which also provided a theoretical basis for gene pyramiding breeding.