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Differential expression analysis of genes involved in high-tempera­ture-induced sex differentiation in the gonads of mature Nile tilapia (Oreochromis niloticus)
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1. College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China; 2. Shandong Institute of Freshwater Fisheries, Jinan 250117, China

Clc Number:

S965

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    Abstract:

    In Nile tilapia, the mRNA expression level of various sex differentiation-related genes can be affected by high-temperature induction during the critical period of sexual differentiation, triggering sex-reversal from female to physiological male. However, it is unknown whether the effect of high temperature on Nile tilapia () sex differentiation-related genes is period-specific. In this study, the expression of eight sex differentiation-related genes, four heat shock protein genes and two methyltransferase genes were in 8-month-old Nile tilapia under high-temperature (36) treatment by quantitative real-time RT-PCR techniques. The results showed that the expression levels of , which are ovary development-related genes, were down-regulated (, which are testis development-related genes, were not significantly changed (>0.05) under high-temperature treatment. The results differed from those for high-temperature treatment of Nile tilapia during the critical period of sexual differentiation. The expression level of DNAJB1 was significantly down-regulated. The expression levels of were not significantly changed in the gonads of females and males under high-temperature induction. Furthermore, it was also found that high temperature significantly up-regulated the expression level of in the female gonad.

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李春歌,赵燕,陈红菊,付佩胜,王慧,季相山. 高温诱导对性成熟尼罗罗非鱼性腺中性别分化相关基因表达的影响[J]. Jounal of Fishery Sciences of China, 2014,[volume_no](2):214-224

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