Cyclins are the positive regulatory subunits of cyclin-dependent kinases (CDK). They play a critical role in the coordination of the eucaryotic cell cycle by binding to the catalytic subunits of CDKs. They share distant sequence homology over a 100 amino acid region called the cyclin box and have been classified into two phylogenetically divergent subfamilies. The first contains A, B, D, E, F, and G cyclins and the second contains the C and H cyclins. Shrimp culture is limited by a lack of methods to breed adults in captivity. This is because female shrimp are unable to reach ovarian maturity without intervention using destructive, expensive, or time consuming methods such as eyestalk-ablation. Thus, there is a need to better understand the of ovarian development. Cyclin E is a cell cycle regulatory factor that plays a very important role in cell proliferation. To better understand the potential function of cell cyclin E in regulating shrimp reproduction, the full-length cDNA sequence of cell cyclin E from black tiger shrimps (denoted as Pmcyclin E) was obtained by high throughput transcriptome sequencing and RACE. The cDNA of Pmcyclin E was 1 706 bp, including a 132 bp 5′-terminal un-translated region (cUTR), a 311 bp 3′ UTR with a poly (A) tail, and an open reading frame (ORF) of 1 263 bp encoding a polypeptide of 420 amino acids with a predicted molecular weight of 47.9 kD and predicted pI of 5.85. The results of Blast and phylogenetic analyses suggest that Pmcyclin E is a new member of the shrimp cell cyclin E family. We measured mRNA expression of Pmcyclin E in nine tissues by real-time PCR. Expression was highest in the ovary and eyestalk, moderate in the intestine, brain, and muscle, and very low in the stomach and . Pmcyclin E expression in the ovary was significantly reduced by injection of MIH, and was induced after eyestalk ablation, suggesting that the gene plays an important role in Our results provide insight into the function of Pmcyclin E in the regulation of ovarian development in the black tiger shrimp.