), a flatfish of deep water species, is a high-nutrition and economic valued species and currently cultured widely in ChinaIn recent years, however, serious germ plasm degeneration occurred due to the lack of long-term and effective broodstock management programsproduction of farmed turbot became highly unstable and total output declined gradually. Thereforethe genetic improvement of turbot will be necessary to sustain the industry development. Within a breeding program in aquacultureThe traits which determines the total harvest yield are highly desirable economic traits, for example, fast-growing, high survival, . The sustainability of turbot culture has been threatened by disease outbreaks and, at present, there is no program in place to minimize such outbreaks. To aid selective breeding for disease resistance traits, in this, study, we conducted a challenge test against using 30 2nd-generation families that were constructed by consecutive selection for faster growth from a base population. We quantified their disease resistance based on survival and tested for differences among families using an odds ratio. There was a significant difference in disease resistance to bacterial infection among families. Twelve families (Family 1, 3, 4, 6, 9, 12, 14, 19, 22, 23, 29, and 30) had high disease resistance with a >65% survival rate; 11 families (Family 8, 10, 13, 16, 18, 20, 21, 24, 25, 27, and 28) had moderately high disease resistance with a 50%–65% survival rate; 3 families (Family 2, 7, and 17) had moderate disease resistance with a 35%–50% survival rate; and 4 families (Family 5, 11, 15, and 26) had low disease resistance with a <35% survival rate. We calculated the odds ratio for disease resistance by b-COX regression analysis, and screened 5 superior families (Family 3, 9, 14, 19, and 23) for third parental generation breeding. The selected families can be used as the core breeding population for turbot resistance to. In addition, the results of the study also provide a reference for selective breeding for disease resistance in cultured turbot.