Improving the efficiency of gene transfer and integration is a key objective in transgenic fish research. To evaluate its use for transgenic fish applications, we used the Tgf2 transposon donor plasmid pTgf2-EF1α-eGFP was re-constructed by replacement of the transgenic elements, EF1α-eGFP with the myosin light chain 2 promoter (mylz2) and the red fluorescent protein (RFP) gene, mylz2-RFP. The recombinant transposon donor plasmid, pTgf2-Mylz2-RFP was intended to serve as a transposon donor plasmid that specifically expresses red fluorescent protein in muscle tissue. The donor plasmid and the transposase mRNA were co-injected into zebra fish fertilized eggs. A total of 972 eggs were microinjected and 803 larvae survived, with a positive rate of 76.6%. The red fluorescence () were subsequently cultivated and 10 sexually mature transgenic individuals were mated with wild-type zebrafish, respectively. F₁ individuals that exhibited red fluorescence on their body surface were obtained from one of the mating pairs yielding an integration efficiency of 10%. The positive rate of F₂ individuals, which were generated by mating the mature transgenic F₁ with the wild-type fish, was 69%. The mature RFP positive F₁ individual was then mated with wild-type fish, yielding a 69% positive rate in the F₂ generation. Our results demonstrate that the transposon improves the efficiency of gene transfer and integration during transgene treatments. Thus, the transposon can be used to construct transgenic fish and in other transgenic fish research.