To explore otolith deposition with alizarin red S (ARS) immersed with conditions of different concentrations (10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L) and the time gradient (8 h, 16 h, 24 h, 32 h), as well as its marking results’ dynamic changes of different relaying days, artificial breeding chum salmon () at the latter period of embryos were researched as immersing objects.This study tried for the protection of chum salmon with the approach of supplement for flag discharges, assessment for the releasing effects and provision for technical reference.The results showed that the sagittal and lapillus otoliths can be marked well, they all had great marked results detected with visible light and green laser light.The visible light marks were gradually weakened with the extension of sampling time, and were almost disappeared at the 80 d. And they were preserved with a long fluorescence intensity under the green laser without any sign of abating. Fitting curves were tried to express the relationship between the marking value and concentration for each series of time points. Combined with security assessments, the suitable group marking conditions for chum salmon otoliths at the stage of embryos were drawed, it was that lying the eggs with ARS immersed at the concentration of 25.9 mg/L to 40 mg/L , the time of 15.6 h to 24 h, and they must satisfy conditions that coordinates under the curve of quadratic function means concentration). The new increased number of grain rounds were significantly correlated with cultured days in linear shape, and correlation equations of sagittal and lapillus otoliths were 0.965 and 0.924, which indicated that from embryos