Immunogenicity of a bivalently-expressed outer membrane protein of Aeromonas hydrophila and Edwardsiella tarda from diseased eels

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Immunogenicity of a bivalently-expressed outer membrane protein of Aeromonas hydrophila and Edwardsiella tarda from diseased eels
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Affiliation:Fishery College, Anguilla Modern Engineering Research Center of Industrial Technology of the Ministry of Education, Jimei University, Xiamen 361021, China
Clc Number:S942
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) were evaluated after they were immunized with a bivalently-expressed outer membrane protein (OMP) of porin II and ompS2 from , respectively. One-hundred and fifty eels distributed into three equal groups were administered ) injections ofA. hydrophila and The plasma collected on days 14, 21 and 28 were used to measure the titers of specific antibodies and lysozyme activity, and whole blood cells collected on these days were used to evaluate the stimulation index (SI). Lysozyme activities in skin mucus and suspensions of liver and kidney were also recorded on these days. On day 28 post-immunization, eels from all three groups were challenged by . Compared with the PBS group, the serum titers of the anti-<0.01) on days 21 and 28. Compared with the PBS and OMP groups, proliferation of whole blood cells in the FKC group was significantly (<0.05) enhanced on day 14, but whole blood cell proliferation in the two immunized groups significantly (<0.05) decreased on day 21 compared with the PBS group. Lysozyme activity in serum, skin mucus, and the liver and kidney suspensions differed significantly (<0.01) between the three groups. The relative percentage survival after challenge with <0.05). These results show that bivalent OMP improved the immune function of the Japanese eel and protected it against infection with . This vaccine has potential for use in freshwater farmed eels.

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郭松林,冯建军,陆盼盼,曹伟棋,赵金平. 鳗鲡病原性嗜水气单胞菌与爱德华氏菌二联表达外膜蛋白的免疫原性[J]. Jounal of Fishery Sciences of China, 2015,[volume_no](1):113-120

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Online: June 23,2015
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