Effects of dietary selenium yeast and tea polyphenols on the activities and mRNA levels of antioxidant enzymes in the liver of juvenile Wuchang

Home > Archive>Volume 22, Issue 2, 2015 >259-268

Effects of dietary selenium yeast and tea polyphenols on the activities and mRNA levels of antioxidant enzymes in the liver of juvenile Wuchang
DOI:
                        
                    
CSTR:
                        [cstr]
                    
Author:
                        LONG MengLONG Meng
Fisheries College, , Huazhong Agricultural University, , Wuhan 430070, , China; Key Laboratory of Freshwater Animal Breeding, , Ministry of Agriculture, , Wuhan 430070, , China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, , W
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
HOU JieHOU Jie
Fisheries College, , Huazhong Agricultural University, , Wuhan 430070, , China; Key Laboratory of Freshwater Animal Breeding, , Ministry of Agriculture, , Wuhan 430070, , China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, , W
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
SU YujingSU Yujing
Fisheries College, , Huazhong Agricultural University, , Wuhan 430070, , China; Key Laboratory of Freshwater Animal Breeding, , Ministry of Agriculture, , Wuhan 430070, , China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, , W
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
WU NingWU Ning
Fisheries College, , Huazhong Agricultural University, , Wuhan 430070, , China; Key Laboratory of Freshwater Animal Breeding, , Ministry of Agriculture, , Wuhan 430070, , China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, , W
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI LiLI Li
Fisheries College, , Huazhong Agricultural University, , Wuhan 430070, , China; Key Laboratory of Freshwater Animal Breeding, , Ministry of Agriculture, , Wuhan 430070, , China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, , W
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHANG WeiranZHANG Weiran
Fisheries College, , Huazhong Agricultural University, , Wuhan 430070, , China; Key Laboratory of Freshwater Animal Breeding, , Ministry of Agriculture, , Wuhan 430070, , China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, , W
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
SUN BochaoSUN Bochao
Fisheries College, , Huazhong Agricultural University, , Wuhan 430070, , China; Key Laboratory of Freshwater Animal Breeding, , Ministry of Agriculture, , Wuhan 430070, , China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, , W
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:Fisheries College, , Huazhong Agricultural University, , Wuhan 430070, , China; Key Laboratory of Freshwater Animal Breeding, , Ministry of Agriculture, , Wuhan 430070, , China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, , W
Clc Number:S96
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Selenium yeast (SY) and tea polyphenols (TP) belong to high-quality natural antioxidants. As an organic selenium source, SP has the effects of both antioxidant and immune-enhancer. Tea polyphenols (TP) is a term for polyphenols in tea, which is helpful to free radicals scavenging, anti-bacterial and anti-inflammatory actions, immunomodulatory effects and so on. In the present study, a two-factorial and three-level experiment was designed to evaluate the effects of dietary SP and TP on selenium deposition in the muscle and liver, the activities and mRNA levels of antioxidant enzymes in the liver of juvenile Wuchang bream, (mean weight 1.75 g±0.01 g). Totally nine semi-purified diets were formulated to provide graded levels of Se (0, 0.25 mg/kg, 0.50 mg/kg) and TP (0, 50 mg/kg, 100 mg/kg). After 8 weeks of feeding trial, the results showed that: (1) Both dietary SY and TP significantly enhanced the weight gain rate (WGR) and specific gain rate (SGR) in juvenile Wuchang bream (>0.05) are there significant interaction effects of dietary SY and TP on WGR and SGR. (2) Dietary SY significantly enhanced selenium contents in the muscle and liver (significant effect of dietary TP on selenium deposition (>0.05), and the interaction effect of dietary SY and TP on selenium deposition was significant in liver (. (3) Both dietary SY and TP significantly enhanced the activities of glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT) by upregulating the expression of these antioxidant enzyme genes. The difference is that SY upregulated mRNA expression of <0.05), whereas TP raised the transcription of of SP and TP has synergistic gene expression significantly and promoting the transcription of . As a result, fish fed the combination diets with SY and TP had higher hepatic total antioxidant capacity (T-AOC) and lower content of malondialdehyde (MDA) than fish in the other dietary groups (<0.05). In light of the above results, it could be concluded that a basal diet supplemented with 0.50 mg/kg SY and 50 mg/kg TP could help keep better antioxidant status by improving the antioxidant enzymes activities and reduced hepatic MDA in the liver of juvenile Wuchang bream.

Key words:selenium yeast; tea polyphenols; Megalobrama amblycephala; antioxidant enzymes; gene expression

Get Citation

龙萌,,侯杰,,苏玉晶,,吴宁,,李莉,张维然,孙博超. 日粮添加酵母硒和茶多酚对团头鲂幼鱼肝抗氧化酶活性及其基因表达的影响[J]. Jounal of Fishery Sciences of China, 2015,[volume_no](2):259-268

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online: June 29,2015
Published:

Journal home

About the journal

Guide for authors

Ethics Statement

Electronic Journal

Subscription

Contact us

Help

Get Citation

Share

Article Metrics

History

Article QR Code