Selenium yeast (SY) and tea polyphenols (TP) belong to high-quality natural antioxidants. As an organic selenium source, SP has the effects of both antioxidant and immune-enhancer. Tea polyphenols (TP) is a term for polyphenols in tea, which is helpful to free radicals scavenging, anti-bacterial and anti-inflammatory actions, immunomodulatory effects and so on. In the present study, a two-factorial and three-level experiment was designed to evaluate the effects of dietary SP and TP on selenium deposition in the muscle and liver, the activities and mRNA levels of antioxidant enzymes in the liver of juvenile Wuchang bream, (mean weight 1.75 g±0.01 g). Totally nine semi-purified diets were formulated to provide graded levels of Se (0, 0.25 mg/kg, 0.50 mg/kg) and TP (0, 50 mg/kg, 100 mg/kg). After 8 weeks of feeding trial, the results showed that: (1) Both dietary SY and TP significantly enhanced the weight gain rate (WGR) and specific gain rate (SGR) in juvenile Wuchang bream (>0.05) are there significant interaction effects of dietary SY and TP on WGR and SGR. (2) Dietary SY significantly enhanced selenium contents in the muscle and liver (significant effect of dietary TP on selenium deposition (>0.05), and the interaction effect of dietary SY and TP on selenium deposition was significant in liver (. (3) Both dietary SY and TP significantly enhanced the activities of glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT) by upregulating the expression of these antioxidant enzyme genes. The difference is that SY upregulated mRNA expression of <0.05), whereas TP raised the transcription of of SP and TP has synergistic gene expression significantly and promoting the transcription of . As a result, fish fed the combination diets with SY and TP had higher hepatic total antioxidant capacity (T-AOC) and lower content of malondialdehyde (MDA) than fish in the other dietary groups (<0.05). In light of the above results, it could be concluded that a basal diet supplemented with 0.50 mg/kg SY and 50 mg/kg TP could help keep better antioxidant status by improving the antioxidant enzymes activities and reduced hepatic MDA in the liver of juvenile Wuchang bream.