We conducted a 90-day experiment in 12 flow-through tanks to evaluate the influence of stocking density on growth performance, muscle composition, and blood biochemical parameters in juvenile hybrid sturgeon [Acipenser baerii (♀)×Acipenser schrenckii (♂)]. Sturgeon (mean total length: 31.55±1.50 cm; mean body weight: 251.11±0.59 g) were randomly distributed into four different stocking density groups of G1 (5.50 kg/m3, 200 fish/pond), G2 (8.27 kg/m3, 300 fish/pond), G3 (11.01 kg/m3, 400 fish/pond), and G4 (13.80 kg/m3, 500 fish/pond). The results show that stocking density had significant effects on the growth and survival of juvenile hybrid sturgeon (P<0.05). Survival rates at the end of the experiment were 99.33%, 97.67%, 95.33%, and 92.80% in the G1, G2, G3, and G4 groups, respec- tively. Final body weight, specific growth rate, and condition factor decreased with an increase in stocking density, whereas the food conversion ratio showed the opposite tendency. Muscle moisture content increased significantly with the increase in stocking density, whereas crude muscle lipid content decreased significantly (P<0.05). However, no sig- nificant difference was observed in ash or crude protein content. Differences between treatments were detected for glu- cose, total protein, and hemoglobin. In conclusion, these results suggest that a high juvenile hybrid sturgeon stocking density could negatively influence growth, muscle composition, and blood biochemical parameters.