). Our objective was to evaluate the epidemiology of the disease and document genotype differences among strains isolated during epidemics at the major giant salamander breeding areas in China between 2010 and 2012. We performed molecular detection, virus isolation and propagation, and viral titer determination. Additionally, the major capsid protein () genes of the virus strains were sequenced and analyzed. The 10 samples that were collected from Shaanxi, Hubei, Hunan, Zhejiang, Jiangxi, and Fujian provinces tested positive. We subsequently obtained 1GSIV epidemic strains genes of the 10 epidemic strains revealed the nucleotide sequences had 99.7–100% similarity and there was little difference among the deduced amino acid sequences. This suggests that all the epidemic strains are of the same genotype. TheGSIVfrogdifferent two branches, but the distanceOur results provide a foundation for the development of a GSIV vaccine.