Cyclin Y is a newly discovered cell cycle-related protein that plays an important role during embryonic development,cell cycle progression and development, and disease. Current research on cyclin Y is very active but no datahave been reported on cyclin Y in crustaceans. Gonads of the black tiger shrimp, , mature if the eyestalk is removed. Thus, the full-length cyclin Y cDNA sequence from (denoted as Pm-cyclin Y) was obtainedusing the rapid amplification of complementary DNA ends method to better understand the potential function ofcyclin Y in the regulation of shrimp reproduction. The full-length cDNA sequence was 1578 bp and contained 108 bp5′ untranslated region (UTR) and a 439 bp 3′UTR. The open reading frame was 1029 bp and coded 342 amino acids(aa). A bioinformatics analysis showed that the amino acid coding sequence had a conserved cyclin box and the homologousprotein box structure domain was 172–257 aa. The predicted molecular weight was about 38.7 kD, and thetheoretical isoelectric point was 6.64. A real-time quantitative polymerase chain reaction analysis detected significantlyhigher Pm-cyclin Y mRNA expression in the ovary than that in other tissues. Pm-cyclin Y mRNA was expressed in theovary at five different developmental stages, and the expression level was highest during phase III. The study abtainedrecombinant expression Pm-cyclin Y in prokaryotes and offered theoretical basis for further research on Pm-cyclin proteinfunction. These results provide a basis for further functional studies of Pm-cyclin Y.