Fish disease has become an increasingly prominent problem in aquaculture development and causessevere economic damage to fisheries. is an important fish pathogen that causes hemorrhagingand septicemia in many cultured fishes. The infection kinetics of WJ-8, that was transformed with aplasmid encoding green fluorescent protein (pEGFPuv-Kar). The purpose of this investigation was to fully elucidatethe pathogenesis of septicemia in blunt-snout bream and provide basic data on pathogenic factors of .Fish were divided into three experimental groups: group A, water temperature 25℃℃GFP μfor 24 h, and bacteria was counted and then transferred to a fish culture tank at afinal concentration of 10CFU/mL. The bacteria bath challenge was maintained for 30 min for groups A and B butnot for group C. Then, fish were transferred to tanks with clean water and the original water temperature wasmaintained. The bacterial counts from the blood, spleen, kidney, gills, intestine, and muscle were detected at 2 h, 4h, 8 h, 12 h, and 24 h after challenge. Bacteria were detected in both infected groups (A and B) but not in the uninfectedcontrol group (C) at all times during the experiment. An increased number of bacteria was observed in thegills of fish in groups A and B (0.05), whereas large numbers of bacteria were also noted in the spleen, kidney,and intestine. There were more bacteria that attached to and proliferated on the fish gills at 32. Thebacterial counts for group B were significantly higher than those of groups A and C (in blunt-snout bream, and high watertemperature is associated with heightened infection risk.