is the largest species of shrimp, with its high economic and natural nutritional value. However, during its breeding process, broodstock breeding technology has been a very important limiting factor. With the development of molecular biology techniques, there are growing concerns about the function of cell cycle genes of crustaceans, especially shrimp. Recently, molecular mechanisms of cell cycle regulatory proteins have been researched. In the study, cell cycle-related protein family genes relating to ovarian development also become a hot topic. Cyclin G2 is a newly discovered cell cycle-related protein that plays an important role during embryonic development, cell cycle progression and development, and disease. Cyclin G2 in some species have been cloned out, but the study of the gene has not been reported in the black tiger shrimp (, mature if the eye stalk is removed. Thus, the full-length cyclin G2 cDNA sequence from ) was cloned by means of rapid amplification of cDNA ends (RACE) approaches to better understand the potential function of cyclin G2 in the regulation of shrimp reproduction. The full-length cDNA sequence was 4075 bp and contained 189 bp 5′untranslated region (UTR) and a 2725 bp 3′UTR. The open reading frame was 1161 bp and coded 386 amino acids (aa) which was highly homology to other species cyclin G2. Bioinformatics analysis showed that the amino acid coding sequence had a conserved cyclin box and the homologous protein box structure domain was 50-150 aa. The predicted molecular weight was about 43.4 kD, and the theoretical isoelectric point was 8.25. The deduced amino acid of cyclin G2 contained 29 phosphorylation sites including 17 Ser, 7 Thr, and 5 Tyr residues. SignalP 4.0 analysis revealed that cyclin G2 did not contain a typical signal peptide sequence. The temporal expression of in different tissues (ovary, heart, intestine, hepatopancreas, brain, muscles, stomach and gills) and different developmental stages of ovary was investigated by Real-time quantitative polymerase chain reaction (QRT-PCR). The lowest expression level of was observed in the hepatopancreas, and the highest in the muscle. During the ovary development stages, was significantly high expressed in stage III ovaries than the other stages. The relative expression of in the ovary was up-regulation after 5-hydroxytryptamine (5-HT) injection, while down-regulation after dopamine (DA) challenge. The study obtained recombinant expression Pmcyclin G2 in prokaryotes and offered theoretical basis for further research on Pmcyclin G2 protein function. Those results offered additional information to understand the development mechanisms of ovaries.