Five male greenhouse reared Chinese soft-shelled turtles ( from the eco-pond for Chinese mitten crabs () (defined as ER) were dissected to collect muscle, soft apron and liver. The proximate composition and amino acid and fatty acid contents in edible tissues were determined to investigate differences in biological indices and nutrient composition of edible tissues. The results showed that the soft apron index and hepatosomatic index of GR were significantly higher than those of ER (<0.05). Muscle crude ash and soft apron moisture contents were significant higher in ER than those in GR (<0.01), while the higher soft apron protein content was detected for GR, but no differences in any other proximate composition factors were detected between in the two environments. The contents of most amino acids, total essential amino acids (∑EAA), and total amino acids (TAA) in muscle and the soft apron of GR were significantly higher than those of ER (<0.05), but higher cysteine content and ∑EAA/TAA ratio were found for ER. The muscle and soft apron essential amino acid scores (EAAS) were similar in ER and GR, but higher mean EAAS was observed in ER than that in GR. C_18:1n9 and C_18:1n7 fatty acid and total monounsaturated fatty acid contents in muscle were significantly higher in GR than those in ER, but C_22:6n3, total polyunsaturated fatty acid (∑PUFA), ∑n-3PUFA, total highly unsaturated fatty acid, and the n-3/n-6 ratio were significant higher in ER than those in GR ( contents were significantly higher in GR than those in ER (<0.05), but no differences were detected in any of the other fatty acids. These results indicate the high nutritional value of male reared in the two environments.