Saline-alkaline water resources are plentiful in China, but fisheries production in these waters is relatively low due to the lack of suitable culture species adapted to different saline-alkaline water characteristics. (Order Cypriniformes and Family Cobitidae) lives in Dali Lake where salinity reaches 6% and alkalinity is as high as 53.57 mmol/L. This species has a high tolerance to high salinity and alkalinity and could be a good aquaculture species for high salinity-alkalinity water. However, little is known about the tolerance limits of cobitid fish compared with those of other freshwater fish species. Thus, the tolerance of three cobitid fish species, such as T. dalaica, to salinity and alkalinity were investigated by 96 h one-way acute toxicity tests and a uniform orthogonal test in this study. The results showed that the 24 h-and 96 h-LC₅₀values for salinity in were 15.64 g/L, 13.58 g/L, and 15.43 g/L and 14.18 g/L, 14.00 g/L, and 11.57 g/L, respectively, with safe concentrations of 4.12 g/L, 4.03 g/L, and 3.74 g/L respectively. The degree of salinity tolerance was The 24 h-and 96 h-LC₅₀values for carbonate-alkalinity in were 117.1 mmol/L, 72.62 mmol/L, and 128.4 mmol/L and 88.83 mmol/L, 155.2 mmol/L, and 120.0 mmol/L, respectively, with safe concentrations of 18.77 mmol/L, 23.66 mmol/L, and 36.30 mmol/L respectively. The degree of carbonate-alkalinity tolerance was The joint effect of salinity and alkalinity was coordinated in and the coordination coefficients increased first and then decreased, but reached the maximum at 48 h. However, the combined effect was coordinated within 0-48 h in whereas it was antagonized during 48-96 h, and the coefficient of antagonism increased gradually with exposure time. These results provide a theoretical basis for promoting breeding and culture of cobitid fish in high salinity-alkalinity water in the future.