An 8-week feeding trial was conducted to determine the effects of different carbohydrate sources on growth performance, body composition, and physiological and biochemical parameters of juvenile Dabry's sturgeon (). Six isonitrogenous and isoenergetic semi-purified diets were prepared with dextrin, wheat starch, corn starch, α-starch, saccharose, and glucose as carbohydrate sources. Mean initial body weight of the study fish was (68.05±1.63) g. The results indicated that feed efficiency and the protein efficiency ratio values of the dextrin, wheat, starch, corn starch, and α-starch groups were significantly higher than those in the saccharose and glucose groups (<0.05). Fish fed the diet containing corn starch had the highest weight gain ratio, specific growth rate, and feed efficiency. No effect of the different carbohydrate sources on condition factor was observed (>0.05). However, the hepatopancreas somatic index (HSI) and visceral somatic index (VSI) were significantly affected by the different carbohydrate sources (<0.05). The highest HIS and VSI were seen in the glucose group, whereas the lowest HIS and VSI were observed in the wheat starch group. The wheat starch group had the highest crude protein content in whole body, muscle, and liver. The glucose group had significantly lower whole-body and muscle crude protein contents than those in the other groups (<0.05). Whole-body, muscle, and crude lipid contents in the dextrin, saccharose, and glucose groups were significantly higher than those in the other groups (<0.05). Hepatic glycogen content was significantly higher in the α-starch group than that in the other groups (<0.05). Muscle glycogen contents in the α-starch and glucose groups were significantly higher than those in the other groups (<0.05). Triglyceride and total cholesterol levels in the liver and serum were positively correlated with whole-body and serum glucose contents. The different carbohydrate sources had significant effects on enzyme activities in the digestive tract and those of pyruvatekinase and phosphoenolpyruvate carboxy kinase in the liver (<0.05). However, hexokinase and phosphoenolpyruvate carboxylase activities in the liver were not significantly affected by the different dietary carbohydrate sources (>0.05). These results indicate that corn starch was the optimal carbohydrate source for juvenile Dabry's sturgeon.