Lymphocystis disease is a viral infection caused by lymphocystis disease virus, which has serious impacts on the aquaculture industry, resulting in heavy losses. In order to select new varieties of Japanese flounder () resistant to lymphocystis disease, eight experimental groups and one control group were established in 2015. The exposure experiment was conducted on one farm with high incidence of lymphocystis disease, and the protection rate of each group was determined. At the same time, the growth traits of each group at 120 d, 180 d, 240 d, and 300 d were measured and compared. The results showed that there were different growth patterns for each group in different periods, showing inconsistent growth patterns among groups. However, in all the groups, the growth performance of group G2 was always ranked the highest for each period. The results also showed that the disease resistance protection rate of the control group was only 59.57%, while the disease resistance protection rate of all the tested groups was above 60%, reaching up to 97.20% for group G2. From the overall analysis, there were no significant differences in the body weight and body length between the disease resistant or sensitive individuals at 390 d of age. The body weight and body length were positively correlated with disease resistance protection rate, while their effects were not significant (>0.05). However, the mean body weight of disease-resistant individuals in group G2 was significantly higher than that of disease-sensitive individuals (<0.05). Based on the group G2, it is expected that a new variety of fast-growing lymphocystis disease-resistant Japanese flounder could be developed.