Spring viremia of carp virus (SVCV) was routinely isolated and identified from tissues of carp () from 40 carp farms in Heilongjiang Province by separation in cell culture and identification using polymerase chain reaction. SVCV isolates were subjected to virus titer determination, phylogenetic reconstruction based on their glycoprotein amino acid sequences and genotyping. Cell culture results showed that the isolates from four different farms infected carp epithelial cells and caused a cytopathic effect. The isolated SVCV strains were designated as Shlj1-Shlj4 and were confirmed as SVCVs by polymerase chain reaction. The titers of SVCV Shlj1-Shlj 4 were 10^6.28, 10^6.88, 10^7.57, and 10^6.38×the 50% tissue culture infectious dose/mL, respectively. The results of the Shlj glycoprotein gene cluster analysis showed that Shlj1-Shlj4 clustered with China strains A2 and BJ0505-2 as well as America strains USA and 212364. The nucleotide sequence similarities of Shlj 1-Shlj 4 with those previously reported SVCV strains were 98.4%-99.8%, and the similarities among Shlj 1-Shlj 4 were 98.6%-99.8%. The highest nucleotide sequence similarity (99.8%) was observed between Shlj3 and USA/212364, and Shlj2 had the lowest similarity (88.0%) with England reference strain 880163. The amino acid sequence alignment results showed that, among the four Shlj strains, the glycoproteins of Shlj 4 contained the most mutations. Genotype analysis showed that Shlj 1-Shlj 4 all belonged to genotype Ia. The study showed that the SVCV detection rate was about 10% in Heilongjiang Province during 2015-2016, and the glycoprotein gene sequences of SVCV strains from different farms varied, indicating that SVCV strains are evolving in the Chinese fish farm environment.