Aging and death are very complex biological phenomena. In recent years, aging has become an important research topic. Researchers have explored the mechanisms of aging at the cellular and molecular levels. is widely distributed in China's coastal areas, with high food, medicinal, and economic value; it is an important cephalopod of economic value in China. It usually dies after spawning, which is a common characteristic of cephalopods. However, the lifecycle and aging-related mechanisms in this squid are still unknown. In this study, we used to examine prohibitin, which is a highly conserved protein that is widely present in eukaryotes from yeasts to mammals and plays an important role in cell cycle management, differentiation, aging, and anti-proliferative activity. gene using rapid amplification of the cDNA end (RACE) technique. The full length of the sequence was 1283 bp, containing a 5'-untranslated region (5'-UTR) of 150 bp, a 3'-untranslated region (3'-UTR) of 236 bp, and an open reading frame (ORF) of 897 bp that was predicted to encode 298 amino acids. Sequence analysis showed that the protein encoded by the gene did not contain a signal peptide, but contained a domain of the PHB family, and the fragment including 20aa to 42aa was a transmembrane structure. The sequence was 87% similar to is clustered with mollusks and arthropods, and the chordate is another, which is in accordance with traditional taxonomy. The expression of S. japonica at different growth stages (larvae, spawning, spawning-and-postpartum). The results showed that the gene was expressed in all four developmental stages. The expressions were lower in the larvae and spawning-and-postpartum stages and relatively high during spawning and spawning. Expressions in brain and liver tissues were high at all stages. The optic gland, an endocrine organ, was associated with reproductive regulation, and its Phb2 gene was significantly decreased after spawning, which indicated that had pleiotropic functions of cycle regulation, controlling cell senescence, and apoptosis. The results of this study provide a basis for further study on the anti-aging effects of Prohibitin2 in and its expression in cephalopods, and also provides some theoretical basis and information for the causes of senility and senescence mechanisms in marine cephalopods.