Changes in the ultrastructure of the carapace during the molt cycle of Macrobrachium nipponense
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1. College of Life Sciences, Henan Normal University, Xinxiang 453007, China;
2. School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, China

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S917

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    Abstract:

    The carapace exoskeletal structure, and its change during the molt cycle of were studied using hematoxylin-eosin staining and the observation of paraffin sections using scanning electron microscopy (SEM). The results showed that the exoskeleton in the intermolt stage (C) is composed of three sub-layers:epicuticle, exocuticle, and an endocuticle, from the outer to the inner layer. The newly formed epicuticle, exocuticle, and endocuticle in the carapace occur in the late premolt D4 and postmolt B stages. The exocuticle is in a strongly basophilic state but the endocuticle is in a weak eosinophilic state. The observation by SEM revealed that, in stage C, both exocuticle and endocuticle have lamellae comprised of parallel sheets of chitin-protein fibers, with abundant pore canals (pc). The stacking density of the lamellae in the exocuticle is higher than that in the endocuticle. The lamellae edges of the exocuticle are regular and the pore canals within them are approximately circular. On the other hand, there are rough edges and unequal-sized, fusiform pore canals in the endocuticle. There is a significant change in the ultrastructure in the newly formed exocuticle after molt. In stage D4,the ultrastructure of the exocuticle is loose, and similar to the old endocuticle from stage C; however, the density of the exocuticle increases in stage A, which may be associated with calcification after molting.

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杜娟,张俊芳,郑征帆,黄亚龙,苗泽龙,宁黔冀. 日本沼虾蜕皮过程中头胸甲外骨骼超微结构的改变[J]. Jounal of Fishery Sciences of China, 2018,[volume_no](2):301-307

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  • Online: April 24,2018
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