The golden cuttlefish ( Hoyle) is one of the most important commercial species in China and Japan. In order to protect and restore cuttlefish resources, researchers have paid a lot of attention to the reproductive biology and artificial breeding techniques of cuttlefish. In the present study, both anatomic methods and tissue section techniques were used to describe the histological and morphological characteristics of oogenesis and ovary development in cuttlefish, in order to elucidate the reproductive physiology of golden cuttlefish. During the oogenesis of , follicular cells were always observed and underwent structural changes before oocyte maturation. The disintegration of follicular cells and the occurrence of yolk substances occurred synchronously. Egg yolk substances accumulated gradually until the oocytes were full and the follicular cells had disintegrated completely, and the nuclei gradually disappeared along with the follicular cells. Eggs were not synchronized and exhibited significantly variable morphology. Immature oocytes adhered to the ovarian matrix and circulated around the matrix of the ovary so that oocytes closer to the center were less developed than those further away. After maturation, the oocytes were free in the ovarian cavity and were discharged through the oviduct. According to the morphology of oocytes and follicular cells and vitellogenesis, the asynchronous oogenesis of could be categorized into five stages, with nine phases:proliferative stage (oogonia phase), small growth stage (free follicular cell phase, single follicular cell phase, and double follicular cell phase), large growth stage (follicle penetration phase, pre-vitellogenic phase, and late-vitellogenic phase), maturation stage, and resorbing stage. Meanwhile, the ovarian development cycle could be categorized into six stages (I-VI), according to ovary appearance and gonad index (GI) and dominant phase of oocytes. Understanding the development and oogenesis of is of great significance to the theory of cephalopod reproductive biology and improving artificial propagation technology.