An 8-week feeding trial was conducted to evaluate the effects of yeast hydrolysate (YH) on the growth performance, serum biochemical indices, digestive enzyme activity, and intestinal morphology of . Four isonitrogenous and isolipidic practical diets (42.5% crude protein and 8.5% crude lipid) were formulated to contain 0%, 1%, 3%, and 5% YH (named Y0, Y1, Y3, and Y5, respectively). A total of 480 , with an average initial body weight of about 1.86±0.01 g, were randomly allocated to four groups with four replicates per group and 30 shrimps per replicate. The results indicated that the shrimp fed the diet containing 5% YH had a significantly higher weight gain rate, specific growth rate, and protein efficiency rate than those fed the control diet (<0.05), and that the lowest feed conversion ratio occurred in the Y5 group (<0.05). Crude protein of whole shrimp significantly increased with increasing YH levels in the diet (<0.05). Dietary supplementation with 5% YH significantly increased the contents of total protein and triglyceride and decreased the activities of aspartate aminotransferase and alanine aminotransferase (<0.05). However, serum glucose and total cholesterol were not significantly influenced by YH (>0.05). Shrimp fed the Y3 diet had higher hepatopancreas trypsin and lipase activities than those fed the control diet (<0.05), but there were no significant differences in amylase activity among all the treatments (>0.05). Shrimp fed the Y3 diet had significantly higher fold heights and widths in their intestines than those fed the control diet (<0.05), and microvillus height in the Y5 group was significantly higher than that in the Y0 group. In conclusion, a 3%-5% YH supplementation in shrimp diet can effectively promote digestion and improve the growth performance, feed utilization, and intestinal morphology of .