The aim of this study was to investigate and analyze quinolone resistance and plasmid-mediated qui­nolone resistance (PMQR) genes in isolated from the commercial aquatic prod­ucts sold in Guangzhou's supermarkets, and to assess the quality and safety of these aquatic products. One hun­dred live tilapias were collected from 14 supermarkets in Guangzhou. The results of high-throughput sequencing analysis indicated that were the dominant bacteria. According to bacterial screening culture methods, 280 strains were isolated from the gills, muscles, and intestinal contents, respectively. All the isolates were evaluated for resistance to enrofloxacin (ENR) and cipro­floxacin (CIP) by agar dilution method. All of the 280 qnr, qepA, aac(6')- genes using PCR. The results showed that 7 (2.50%) isolates were resistant to ENR, while 6 (2.14%) isolates were resistant to CIP. The resistance rates of ENR and/or CIP in isolates obtained from the muscles were much lower than those in the microorganisms isolated from the gills and intestinal contents. All the isolates obtained from different issues were much more resistant to fluoroquinolones than the isolates, 59.89% isolates harbored PMQR genes, and 5 types of PMQR genes were detected, including Aeromonas isolates, 6.79% harbored PMQR genes, and only were detected. Only a few resistant bacteria were screened in the muscles, which form the edible part of the tilapia; so, the food products were considered relatively safe. However, resistant was predominantly isolated from the intestinal contents and gills, and most of the isolates carried various types of PMQR genes, suggesting a potential risk of drug-resistant transmission from these tissues. Therefore, it is important to strengthen monitoring of antimicrobial resistance of bacteria in aquatic products.