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Temporal-spatial characteristics of lipea gene expression in Cyprinus carpio and its correlation with fat deposition
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1. Key Laboratory of Freshwater Fishery Germplasm Resource, Ministry of Agriculture and Rural Affairs;Shanghai Ocean University, Shanghai 201306, China;
2. National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China

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S965

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    Abstract:

    The objectives of this study were to investigate the expression pattern of Cyprinus carpio), to lay the foundation for studying and understanding the molecular regulation mechanism of fat deposition in common carp. In this study, a full-length cDNA sequence of gene in common carp was cloned by reverse transcription PCR and rapid-amplification of cDNA ends (RACE) technology, and the expression pattern of gene in different tissues of common carp and during its different developmental stages. cDNA of the gene of common carp was 3379 bp, with an open reading frame of 2079 bp, 5' untranslated region was 230 bp, 3' untranslated region was 1067 bp, encoding 693 amino acids. Multiple sequences alignment revealed that the amino acid sequences of Paralichthys olivaceus, and , respectively. It was determined that the sequence differences were mainly concentrated on the C-terminal polypeptide sequence between different species, and the structural analysis of proteins showed that the protein contains three functional domains with lipase activity, acetyl hydrolase activity, and hydrolase activity, respectively. Real-time quantitative PCR showed that the gene was expressed in all tested tissues, and the relative expression level was the highest in visceral fat, followed by abdominal muscles, and lowest in the blood. Its expression declined during the development of embryo; it was the highest in the 8-cell stage, followed by the late blastula stage. It was the lowest in the feeding period. The mRNA expression in different fat tissues negatively correlated with adipose tissue in the abdominal cavity; the highest and the lowest expression levels differed significantly (<0.01). The expression also positively correlated with dorsal muscle but it was not significant ( gene highlights its function and molecular regulation mechanism of fat development in common carp.

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党红阳,吕伟华,孙志鹏,匡友谊,曹顶臣,孙效文,郑先虎. 鲤lipea基因表达的时空特征及与脂肪沉积相关性分析[J]. Jounal of Fishery Sciences of China, 2019,[volume_no](2):251-260

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  • Online: March 27,2019
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