is a common conditional pathogen in aquaculture, which is widely distributed in the world. Rapid detection of the pathogen is necessary for the prevention and control of the diseases caused by the bacterium. Aptamers exhibit great advantages in the detection of microorganisms owing to their high affinities and specificities. In the present study, was detected based on its aptamer and its selection products following various steps, such as binding, washing, separation, amplification, and electrophoresis. The results showed that the aptamer and the selection products could superiorly detect and its inactivated form. The lowest detection limits were 10 cfu/mL of cfu/mL of inactivated by the selection products. The system of detection had better affinity and specificity for from other aquatic pathogens such as , and therefore shows good application potential in the detection of pathogens in aquaculture.