Glyptosternum in China. It is only distributed in the Yarlung Zangbo River in Tibet. To determine the existence of morphological variations and the degree of differentiation between two wild groups, fish from the Nyang River, a tributary of the Yarlung Zangbo River and Shigatse river section, a main stream, were evaluated. Here, 10 morphological parameters and 19 structural parameters were assessed. The results were as follows:the independent sample -test revealed differences in external morphological characteristics between the Nyingchi and Shigatse groups. Principal component analysis revealed that the diversity of morphological characteristics in the Shigatse group was higher than that in the Nyingchi group, and it was difficult to completely separate the two groups based on morphological characteristics alone. Moreover, the morphological differences in the Shigatse group were diverse, and the measured traits were insufficient to summarize the morphological differences between the two groups. The difference coefficient showed that the apparent morphological differences between the two groups did not reach the subspecies level. Environmental differences and different survival pressures may be the main causes of morphological differentiation between the two groups, which may have been inhibited by extensive gene exchange between previous groups.