China has become the largest producer of sturgeon in the world. Hybrid sturgeon[)] is one of the dominant aquacultures organisms in China. However, the feeding strategy for hybrid sturgeon has not been clearly outlined. To determine the optimum feeding rate and frequency for juvenile hybrid sturgeon, an experiment was conducted to study the effects of different feeding rates and frequencies on the growth performance, body composition, and biochemical parameters of juvenile hybrid sturgeon. The fish were randomly assigned into 10 groups with 3 replicates per group, including 5 groups (initial body weight:335.87±0.82 g) treated with different feeding rates (0.4%, 0.8%, 1.2%, 1.6%, and 2.0%) and another 5 groups (initial body weight:334.77±1.06 g) treated with different feeding frequencies (once, twice, three times, four times, and five times a day). After the 10-week feeding trail, the increased feeding rate significantly improved the weight gain rate (WGR), specific growth rate (SGR), hepatosomatic index (HSI), viscerosomatic index (VSI), and condition factor (CF) of hybrid sturgeon (<0.05). In addition, the crude protein and lipid content in muscle, crude lipids in the liver, pepsin activities, serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST) also increased significantly with the increasing feeding rate (<0.05). However, the feed efficiency (FE), whole body moisture, and muscle moisture decreased significantly with the increasing feeding rate (<0.05). Meanwhile, the whole body crude lipids, total protein (TP), albumin (ALB), and high density lipoprotein cholesterol (HDL-C) in the serum firstly increased with the increasing feeding rate, but then decreased (<0.05). On the other hand, the feeding frequency had no significant effects on the WGR, SGR, or FE of hybrid sturgeon after the 10-week feeding trail (>0.05). The VSI, CF, and content of whole body crude lipids of the fish fed once a day were significantly lower than that of the other groups (<0.05). In addition, a significantly higher content of the whole body crude protein was observed in the fish fed twice a day compared with that of the other groups (<0.05). No significant effects of the feeding frequency were found on the digestive enzyme activities in the stomach and intestine or the serum biochemical parameters, except for the glucose level (>0.05). Overall, according to the results of this study, the optimum feeding strategy with regards to the feeding rate and feeding frequency for juvenile hybrid sturgeon (300-600 g) was suggested to be 1.2%-1.6% of the body weight with meals twice per day.