Abstract:Brachymystaxlenoktsinlingensiswerecaughtfromthewildand,afterartificialfertilization,embryonicandlarvaldevelopmentwereinvestigated.Larva)Thetotalaccumulatedtemperatureofembryonicdevelopmentwas19datawatertemperatureof9.31edefinedsixbroadsofembryogenesis(zygote,cleavagestage,blastulastage,gastrulastage,neurulastage,andorganformationstage),whichminorperiods.Thehatchinglarvaehadatransparentbodywithclearlyvisiblesarcomeres,butwithlimitedswimmingcapability.Fivedaysafterhatching,apatchofmelaninappearedonthebodysurface.Theyolksacbegantodisappear12daysafterhatchingandparrmarkingsstartedtoappearafter46days,atwhichstagethefinsofthelarvaeresembledthoseofyoungfish.Themorphologicalcharacteristicsoftheembryosandlarvaearedescribedandthefactorsaffectingontogenesisoftheearlylifestagesof,includingwatertemperature,dissolvedoxygenandwaterquality,arediscussed

Abstract:betweenlateembryoandlarvalstageswasstudiedbymicroscopicobservationofserialsections.Atawatertemperatureof10.0–16.2°C,theorderofappearanceoflymphoidorganprimordiawasthymus,headkidneyandspleen.Theprimordiumofthethymusformed5dbeforehatchingandsmalllymphocytesappeared1dbeforehatching.Nineteendaysafterhatching,connectivetissueandbloodcapillariespenetratedintotheparenchymaofthethymusandthetrabeculaeappeared.Differentiationintoindistinctinnerandouterzonesoccurredafter35d.Theheadkidneyprimordium,whichconsistedofundifferentiatedhemopoieticstemcells,appeared2dbeforehatching.Kidneytubuleswereformed3dafterhatchingandlymphoidorgansafter10d.Between35dand40dafterhatching,theepitheliumoftherenaltubulesbegantodegradeandbloodsinusesappearedintheheadkidney.Whentherenaltubuleshaddegeneratedcompletely50dafterhatching,theheadkidneywascomposedprincipallyoflymphoidtissueandalargenumberofbloodsinuses.Thespleenprimordiumappeared5dafterhatchingandtheorganbecamelymphoidonthe16thday.Capillariesandbloodsinuseswereformedinthespleenbetween35dand50d.Weconcludethattheimmuneorgansaredifferentiatedearlierinthaninotherfishes.Althoughtheoveralldevelopmentratewasrelativelyslowin

Abstract:volutionofdifferentecologicalgroupsAfter1forownloadmaximumlikelihood(ML)andneighbor-joiningtreesaThiswasfollowed1sKH,SH,ELWand2sKHProrocentrumProrocentrumWeconcludethatsubstantiallyreflectstheevolutionofdinoflagellateslaidafoundationforfurtherresearchontheevolutionbetweendifferentecologicalgroupsofdinoflagellatefromthegeneticlevel.

Abstract:bottomtrawlsurveysconductedintheEastChinaSeaduringSeptember2010andMay2011,thispaperpresentsbeakmorphologytherelativestabilityofindices,mantlelength,andrelativegrowthrostrumlength(RL)crestlength(CL)andwingwidth(WW)HoodbyBeaklengthindicesRL/HL,RL/CL,HL/CLremainedverystable,anddidnotalterobservablywithchangesinmantlelengthorbodyweight.Theseratioscouldbeusedasstable.

Abstract:-ATPaseisaubiquitousplasmamembraneenzymethatandItsfunctioniscoupledtothehydrolysisofATPandNaplaysacentralroleincellioncontent,ofcells.S-subunitcontainsanintracellularATPbindingsite,aphosphorylationsite,andanextracellularbindingsiteforthemaintainingstabilityandcorrectmembraneorientation-subunithavebeenidentifiedinmammals.-subunitisexpressedubiquitouslyamongtissuesandisthoughttoplaya‘‘housekeeping’’roleinmaintainingNacellviability-ATPaseefull-lengthcDNAencoding+wasclonedfromusinghomologycloningandRACEPCR.The1subunitandthoseofother-ATPasehadhighsimilarity(91.97%Rhabdosargussarba,XenopuslaevisReal-timegenewastissues:brain,liver,kidney,intestines,spleen,andgills.-ATPasebrain,,andthe

Abstract:Charybdisjaponicaincludingclusteranalysis,principalcomponentanalysis,anddiscriminantanalysis.Fourwildpopulations,locatedinDalian,LaizhouBay,HaizhouBay,andXiangshanBay,weresurveyed.Thereweresignificantdifferencesbetweenmaleandfemalepopulations).ClusteranalysisrevealedthattheDalianstockwasmostcloselyrelatedtotheLaizhouBaystock,andthattheXiangshanBaystockwasmostdistantlyrelatedtotheLaizhouBaystock.Thecumulativevariancecontributionratio,intermsofthefiveprincipalcomponents,wasformalepopulations..TheaccuracyratewaslowestfortheDalianstock()andhighestfortheXiangshanstock(100%).Thetotalaccuracyrateofdiscriminantanalysesonmalepopulationswas).Alloftheanalysesindicatedthatthereweremorphologicaldifferencesamongthefourpopulationsof

Abstract:Aristichthysnobilisisahybridthegrowthperformanceincagehecombiningability,over2years,hatchingrateoftheiroffspringwas84.93%,andthemalformationratewas4.09%.Atotalof13000frywereobtainedinthesecondyear.Titsfemaleparent,butTwentysixmorphologicalfeaturesweremeasured,23ofwhichresembledthefemaleparentandthreeofwhichtendedtowardsthemaleparent.Cofthemajordiameteroferythrocytetriploid:inhybridgroupsresearchaimedtoprovideandpracticeof

Abstract:Litopenaeusvannameiaterqualityparametersweremeasured,includingchlorophyll-a(Chla),watertemperature(T),pH,dissolvedoxygen(DO),transparency,totaldissolvedsolids(TDS),chemicaloxygendemand(COD),nitrogen(TN)andphosphorus(TP).Intotal,107groupsofmonitoringdatawereobtainedandcompiledasdescriptivestatistics.MultiplelinearregressionfollowingprincipalcomponentanalysiswasusedtosimulatetherelationshipbetweenwaterqualityvariablesandChlaincultureponds.Basedonthecriterionofeigenvaluesgreaterthan1,fourlatentvariablesoccupied77.56%tothecumulativecontributiontovariance.SubsequentmultiplelinearregressionanalysisshowedthatalatentvariableCwereclearlypositivelycorrelatedwithchlorophyll-a:Mn.Phosphoruswasthelimitingfactorfortheprimaryproductivityoftheponds.Weconcludethatorganicsandphosphorusarethemainfactorsthatneedtobefocusedontocontroleutrophicationin

Abstract:Apostichopusjaponicus1thebacterialstrainswerepreliminarilyselectedbyevaluatingthetheseacucumber.tomedium,withultureconditions℃Aftersisolate(wereabletodegradethefeedNremovalratesof95.0%,.biochemicalanalyses,anPseudomonasconcentrationsoftheintheliquidseacucumber-feed-mediumacterialcultivationtimehadasignificanteffectonthefeed-degradingactivitiesofthethreestrains(sof,strainDR8wasconsideredtobethe<0.05mixturesofthefeed-degradingabilitiesofthebetterthanthatofthesinglestrain.3low-temperatureorganic-pollutant-degradingbacteriascreenedinthisstudyforpollutioncontrolinseacucumberculturepondsandthedevelopmentofprobiotics

Abstract:kineticsofinhibitionoftrypsin-likeproteasefromrreversiblewere0.155mmol/Land8.44μmol/(L·min),respectively.AstheconcentrationofSTIwasincreased,theactivitydecreasedwithICof2.8μg/mL.STIslowly,reversiblyandcompetitivelyinhibitedtheenzymeactivityatlowconcentration.Therateconstantoftheforwardinactivation(,whichwasaboutfivetimeshigherthanthevalueof0.039).Therefore,atsufficientlyhighSTIconcentrations,theenzymewouldbecompletelyinactivated..

Abstract:

Abstract:maybeproducedbyvariouskindsofbacteria.Theyareusuallycomposedofglycoprotein,polysaccharide,celluloseandnucleicacid.AsMBFcanbeeasilybrokendownbymicroorganismsintheenvironment,theyhavepotentialapplicationinthesewagetreatmentindustry.playanimportantroleintheconversionoffoodenergyandinthemaintenanceof(hereinafterreferredtoas1102)fromrDNAsequenceanalysisandgaschromatographicwhole-cellfattyacidanalysiswereemployedtoidentifythebacterium1102.Toanalyzeitsflocculatingactivity,theMBFwasextractedandpurifiedfromthefermentationbrothofbacterium1102thathadbeenfermentedinGauze’smediumNo.1amyloliquefaciens1102was80.19%.ThehighestyieldofMBF)wasobtained72hafterinoculating.bygelpermeationchromatography,phenolsulfuricacidcolorimetry,andautomaticaminoacidanalysis,respectively.TheaveragemolecularweightofMBFwasabout7thepolysaccharidecontentwas58.58%,andtheaminoacidcontentwas2.49%.inmanycountriesandshowsagreatpotentialforinincreasingfoodconversionrateandforzerowaterexchange.BFTusuallydependonmicroorganismsthatareabletoproduceMBFintheculturesystem.Bacterium1102ishighlyefficientforproductionofMBFathighflocculationratesandcanbeusedinmarineaquaculturetoflocculateorganicdetritusandmicroalgaeinponds.Inaddition,bacterium1102isahelpfulbecausemanyspeciesofbacillusareprobioticforaquaculture.

Abstract:Procyprisrabaudi(Tchang)weredeterminedinthelaboratorybythestomachcontentmethod.Duringtheexperiment,thefishwerefedonThefeedingintensityexhibitedadistinctcircadianrhythm,withasinglepeakbetween00:30and06:30.Gastricevacuationratesofjuvenilecarpofmeang.Linear,exponentialandsquarerootmodelswerefittedtothegastricevacuationdataandallthethreemodelsprovidedagoodfittothedata0.1692usingthismodel,thegastricevacuationratewascalculatedtobe0.169g.Thedailyfoodconsumptionwasmeasureddirectlyas24.91.UsingtheElliott-Persson(1978)model,thefoodconsumptionofjuvenilerockcarpintheirnaturalenvironmentwasestimatedas.Tosimplifyquantificationofthedigestivetractcontentsofrareandendangeredfishwithcomplicateddigestivetracts,weemployedanempiricalformulatocalculatetheweightoftheEDW=0.24(BW/BL)).Thisstudyprovidesbasicdataforquantitativeresearchonthefeedingecologyandenvironmentalcarryingcapacityofrockcarp.

Abstract:Euphausiasuperba4,thedragresistanceofthetrawlwashigherandthewidthofthenetmouthwasgreater.(2)Ata/=0.45,thesefactorsinfluencedresistance(Fs)andpowerconsumption(Ps)inthefollowingorder:gravity(A)>crossrope(C)>(B).Theorderoftheirinfluenceonthenetmouthheight(Hs)wasA>B>C,andonenergydissipation(Ce)wasC>A>B.TheoptimalcombinationforhydrodynamicperformancewasA3B3C1.At=0.5,theorderofinfluenceonFs,HsandPswasC>A>B,theorderoftheirinfluenceonCewasB>C>A,andtheoptimalcombinationforhydrodynamicperformancewasA2B3C3.At=0.55,theorderofinfluenceonFsandPswasC>A>B,theirinfluenceonHswasB>A>C,theirinfluenceonCewasB>C>A,andtheoptimalcombinationforhydrodynamicperformancewasA2B3C3.(3)WhenL/Swasextremelylow(0.2),thehorizontalexpansionofthenetmouthwasapproximately23m.Althoughthewidthofthenetmouthwasgreatlyincreased,thetrawlwasinefficientoverallandtherewasasignificantincreaseinthecoefficientofenergydissipation.(4)Themainmethodofadjustmenttoimprovetheoverallperformanceofthetrawlistoincreasetheeffectofgravitybyappropriatelylengtheningtheandthecrossrope.Basedonthisstudy,toachieveahighexpansionofthetrawlatlowtowingspeeds,werecommendtheuseofhighlyexpandableotterdoors.

Abstract:themiddlereachesofHeilongjiangRiverinMay(spring),July(summer)andThedynamicsofphytoplanktondiversitywereexaminedandwaterqualityanalyzedusingaterethods.Thespeciescompohighestinthemiddlereachesoftheriver.Phytoplanktonwereabundantandwithhighdiversity(thevalueofwas0.83–0.88).Thelowestvaluesof()inthespring.Thediversityofphytoplanktonrevealedseasonaldrayrelationalanalysisindicatedthat0.879andrayrelationalanalysiswithwithintheOSstandard‘River,andthatthewaterqualitywaslevelI–IIof.Therivercouldalsoprovideconditionsforthe

Abstract:tuna()distributioninfishinggroundsinthetropicalIndianOceanandthermoclinecharacteristics,calculatedfrommonthlytemperatureprofiledatafromArgobuoysandthemonthlyCPUE(catchperuniteffort)frombigeyetunalonglines.Seasonalvariabilitywasobservedinthedepthoftheupperboundarytemperatureandinthelowerboundarydepth.Ouranalysissuggestedthatthedistributionofbigeyetunawasrelatedtotheseseasonalvariationsofthethermocline.TherangeoftheupperboundarydepthforhighCPUEduringthesummermonsoonwas30–50m,whichwaslowerthaninthewinter(50–70m).AhigherCPUEwasfoundfromnorthernMadagascartoSomaliawherethelowerboundarydepthwasat170–200m.Wherethelowerboundarydepthwasmorethan300m,thevalueofCPUEwaslow.TherangeofupperboundarytemperaturesinthehigherCPUEfishinggroundswas27–30mduringthewintermonsoonand24–30mthesummermonsoon.Frequencyanalysisandtheempiricalcumulativedistributionfunction(ECDF)wereusedtocomputetheoptimumrangeofthermoclinecharacteristics.Theoptimumdepthrangeoftheupperboundarytemperaturewas26–29mandtheforthelowerboundarytemperature,itwas13–15m.Theoptimumrangeoftheupperboundarydepthwas30–60mandthelowerboundarydepthwas140–170m.TheconclusionswereconfirmedusingKolmogorov-Smirnovtest.inthecentralfishinggroundofbigeyetunawerepreliminaryobtainedinthispaper.Theresultscouldpro

Abstract:Thecommunitystructureandbiodiversityofphytoplanktonwereinvestigated,andwaterqualitywasassessed,infourreservoirswithdifferenttrophicstates,inHubeiprovince,between2006and2008.Therewere215speciesofphytoplanktoninthefourreservoirs,rankedinorderofabundance:diatoms,Chlorella,andblue-greenalgae.Blue-greenalgaeoccurredprimarilyinthelayerabove,diatomsweremostabundantinthelayerbetweenSDand2SD,whereasotheralgae,including,weremainlyfoundinthelayerabove2SD.thanTheaveragedensityandcellsLand2.740mgand3.088mginTaoyuanheReservoir.Thedistributionofphytoplanktonbiomassamonglayerswasgenerallycharacterizedas:SD>surface>2SD>3SD>bottomexceptfortheDaoguanheReservoir,inwhichtheorderwassurface>SD>2SD>3SD>bottom.Therewasahighlysignificantdifference()andthePielouevennessindex(eservoirandtheother0.05)amongDaoguanhe,Taoyuanheandhereservoirs.Anegativecorrelationwasfoundbetweentotalnitrogenandphytoplanktonbiomass,ositivecorrelationexistedbetweentotalphosphorusandphytoplanktonbiomass.Variationsofphytoplanktoncommunitystructurewereprimarilyattributedtothedifferencesinthetrophiclevelsofthefourreservoirs.

Abstract:)fromtheOysterSelectionBreedingProgramwerecompared.Oysterswereharvestedat360daysofagein2009andin2010andcomprised24and32full-sibfamilies,respectively.Alloystersinbothyearclasseswererearedunderthesameconditions.Shellheight,shelllength,shellwidth,totalweight,shellweight,meatweightandmeatratio(100%×meatweight/totalweight)weremeasuredin30oystersfromeachfamily.Growth-relatedtraitsshowedsignificantdifferencesbetweenthetwoyearclasses(<0.05).Therewasatendencyforoysterssampledin2009tohavelowershellheights,meatweightsandmeatratiosthanin2010,buttheirtotalweightswerehigherin2009.Exceptforthemeatratio,therewasasignificantpositivelinearcorrelationbetweenshellheightandtheothergrowth-relatedtraitsinbothyearclasses()and0.27±0.13,respectively.Allphenotypicandgeneticcorrelationsbetweentwogrowth-relatedtraitswerepositive.Therewasalsoadifferenceinthedegreeofcorrelationbetweentwotraits.Theuseofgrowth-relatedtraitsinbreedingprogramsdesignedtoimprovegrowthisdiscussed.

Abstract:)belongtothenuclearreceptorsuperfamily,whichregulatesthebiosynthesisandmetabolismoflipids,andthedifferentiationandformationoflipocytes.)wasclonedbyRT-PCR.Sequencehomologyanalysisshowedthatrainbowtroutgenewassimilartothosereportedinothervertebratespecies.ThemRNAsequenceandthededucedaminoacidsequenceofrainbowtrout%–93.8%,respectively,withthoseofothervertebrates.TheDNA-bindingdomainandtheligand-bindingdomainofPPARαhavebeenhighlyconservedintheevolutionfromfishtohumans,whichshow88.0–99.6%aminoacidsequenceidentities,respectively,withthoseofothervertebrates.Phylogeneticanalysisindicatedthat)belongtothesamebranchofthephylogenetictree.Usingquantitativereal-timeRT-PCR,weobservedthatrainbowtroutmRNAwasexpressedpredominantlyinlipid,muscle,ovarian,renal,andintestinaltissues.

Abstract:experimentreportedtheeffectsofageongrowth,survivalandpearlproductiontraitsofpearloyster.Thedonorandhostoystersweresampledeachfromtheselectedlinebyatwosuccessiveselectionforsuperiorgrowthinthebasepopulation.Pearlproductiontraitsincludedretentionrate,pearlthickness,highqualitypearlrateandpearlyield.Fourgroupsweredesignedinthepresentexperiment:Agroup,2-year-oldindividualswithnuclei-operation;Bgroup,2-year-oldindividualswithoutnuclei-operation;Cgroup,1.5-year-oldindividualswithnuclei-operation;Dgroup,1.5-year-oldindividualswithoutnuclei-operation.Growthtraitsofthefourgroupswereevaluatedbasedonthetotalweightmeasurementdataatdays15,30,60,180,270and450afternucleus-insertingoperation.Survivalratewereevaluatedamongthefourgroupsateachsamplingtimeandretentionrate,pearlthickness,highqualitypearlrateandpearlyieldbetweengroupsAandCwerealsocomparedatdays270and450.Itwasfoundthatthereweresignificantdifferences15,30,60,180,270and450afternucleioperation(higherthant<0.05).Thegrowthratesofly<0.05).Atday270afternucleus-insertingoperation,retentionrate,pearlthicknessandpearlyieldofgroupCweresignificantlyhigherthan<0.05),whilethedifferenceinhighqualityratebetween>0.05).Atday450afternucleus-insertingoperation,retentionrate,pearlthickness,highqualitypearlrateandpearlyieldofgroupCweresignificantlyhigherthan<0.05).Thepresentresultssuggestanewculturemethodforimprovingpearlqualitybysamplingyoungoysterashostoyster.

Abstract:bindingratesofdifloxacin(DIF)toplasmaproteinsofandtoinvestigatetheeffectsoftemperature,andoftheconcentrationsofplasmaandthedrug,onthebindingcharacteristics.Thebindingratedecreasedfrom32.4%to19.6%asthedrugconcentrationwasraisedfrom0.5μg/mLto5μg/mLbutwasunchangedbetweendrugconcentrationsof5μg/mLand40μg/mL.Thebindingrateswerenotaffectedbytemperaturesof4,15,20,25,or37,butthebindingrateinundilutedplasmawassignificantlyhigherthanintwofold(8.87±0.39)%andfourfolddilutedplasma()anddissociationconstant(mol/gand4.04×10mol/L,respectively.Theconcentrationofthedissociateddrughadalinearrelationshipwiththetotalconcentrationofthedrugandwasgivenbytheequation:Thus,thebindingrateofDIFtoplasmaproteininisdrugconcentration-dependent;itisnotaffectedbyexperimentaltemperaturesbetween4;itislinearwithconcentrationsofDIFbetween5μg/mLand40μg/mL;anditshowsanonlinearrelationshipbetween0.5μg/mLand5μg/mL.