Abstract:Thepituitarygonadotropins(GtHs),follicle-stimulatinghormone(FSH)andluteinizinghormone(LH)regulatesteroidogenesisandorange-spottedgrouper()wasfirstdividedintosevenphases(phaseItophaseVII)accordingtothehistologicalcharacteristicsofoocytes,andthetemporalexpressionpatternsofFSHRandLHRwerefurtherdetectedinparallelwiththeexpressionprofilesofadownstreamfactor(Cyp19a)duringdifferentdevelopmentalphasesoftheovary.Quantitativereal-timePCRdeterminedtheabundanceofFSHRwasatarelativelylowlevelwithin,thenelevatedgraduallyinphaseIII,significantlyincreasedinphaseIVandphaseV,andremarkablydecreasedinphaseVI.Contrastingly,thetranscriptsofLHRremainedatalowlevelduringearlydevelopmentoftheovary(phaseItranscriptionalphaseIII,butsignificantlyincreasedinphaseIVandphaseV,andthensharplydecreasedinphaseVI.Generally,Cyp19ahadasimilarexpressionprofilewithFSHR.Insummary,ourresultssuggestthatbothFSHRandLHRareinvolvedintheregulationofovarydevelopment,butplaydistinctrolesinthedifferentdevelopmentphasesoftheovary.FSHRmaybethemajoractivatorofCyp19atranscription.ThisinvestigationcontributestoelucidatetheactionsofGtH/GtHRsignalsystemsinreproductiveregulationintheorange-spottedgrouper.

Abstract:changesofandsalinityof30±1withinalaboratory.Theresultsshowedthatembryonicdevelopmentgoesthroughninestages:fertilizedegg,cleavage,blastula,gastrula,naupliusdevelopment,eyepigmentprophase,eyepigmentanaphase,pre-hatchingandhatchingstages.Thereisnosignificantchangeinembryosizeduringearlydevelopmentalstages.However,atthepre-hatchingstage,theembryosbecomesignificantenlargedfrom0.6mm.Thecoloroftheembryograduallychangesduringdevelopmentfromlightgreen,darkgreen,darkyellow,totawny.Thetotalincubationperiodisapproximately13daysatawatertemperatureof

Abstract:withSSRmarkersforJapaneseflounder(681383Bisthemaleparentand6812E36isthefemaleparent.ThelengthcM,.ThelengthcM,laidthegroundworkandeffectivelypromotethegeneticimprovementofParalichthys,makeflounderaquaculturesustainabledevelopment.

Abstract:progeniesofthemappingfamilybyusing320screenedSSRprimercombinationscontaining112segregationdistortionmarkers(<0.05).Thefemalelinkagemapincluded21linkagegroupsand242pairsofSSRmarkersspanningcMandtheobservedcoveragewas83.3%.Themalelinkagemapincluded21linkagegroupsandpairsof218SSRmarkersspanning15.5cMandtheobservedcoveragewas82.0%.ThegeneticlinkagemapsareverysignificantforQTLanalysesmarker-assistedselection(MAS)breedingprogramsforeconomicallyimportanttraitsandsexcontrolin

Abstract:isaneconomicallyimportantbreedingcrabfoundalongthesoutheastcoastofChina.Althoughextensiveresearchonreproductivephysiologyhasbeencarriedoutforthisspecies,descriptionoftheregulatorymechanismofthegonaddevelopmentatamolecularlevelislimited.Withinalltissues,themajorityofintracellularproteinsaredegradedbytheubiquitin(Ub)–proteasomepathway(UPP).Inthispathway,UbisconjugatedtoproteinsthataredestinedfordegradationbyanATP-dependentprocessthatinvolvesthreeenzymes.Todate,somestudieshavediscoveredthatcomponentsoftheubiquitinsystemmaybeinvolvedinvariousstepsandprocessesduringgametogenesis,modulationofsteroidreceptorconcentrations,placentaldevelopmentandendometrialmodificationatthebeginningofpregnancy.However,researchontheactionofUPPingonaddevelopmentincrabspeciesUbgene()wasidentifiedandcharacterizedusinganapproachwhichcombinestheexpressedsequencetag(EST)methodandrapidamplificationofcDNAend(RACE).Theexpressionprofileofinthevarioustissuesanddifferentdevelopingstagesofthegonadwasdeterminedbyreal-timequantitativePCR.ThefulllengthcDNAofthegeneisof555bp.Itencodesaproteinof154aminoacids,inwhichthe1-76aminoacidsfallwithintheubiquitindomain,andthe101147aminoacidsfallwithintheribosomal_S27adomain.ThesecondstructurepredictionrevealedthattheubiquitindomainoftheSp-Ubproteincontainsfiveextendedstrands,anα-helixandsomerandomcoils.Moreover,thethree-dimensionalstructurepredictedbytheSWISS-MODELshowedthatitcontainsanα-helixandfourβ-pleatedsheets.AlignmentoftheaminoacidsequenceswithindifferentUbproteinsindicatedthattheubiquitindomainandtheribosomal_S27adomainamongdifferentspeciesarehighlyconservedduring.Real-timequantitativePCRresultsshowedthatwasexpressedbytheubiquitindomaininalltissuesexamined(P>0.05)whichillustratedthattheUbgeneplaysawidevarietyofrolesinbiologicalprocesses.TheexpressionprofilesofinthedifferentdevelopingstagesoftheovaryrevealedthatitsexpressionlevelwasthehighestatstageO5followingstageO1,O4andO3.ItwasthelowestlevelatstageO2whichwassignificantlylowerthanstageO5(P<0.05).Withinthedevelopingstagesofthetestis,theexpressionlevelofwashigherinstageT2followingstageT1andT3,anditsexpressionlevelwassignificantlyhigherinstageT2comparedtostageT3(P<0.05).Duringgametogenesis,theoogoniaandspermatogoniumundergoactivemitosisproliferationforincreasingtheirnumberattheproliferationstage.Previousresearchshowedthatcyclin/Cdksworkcloselywiththeubiquitin-proteasomepathwayduringthemitoticcycle.Thus,itisreasonabletoobservethattheexpressionlevelofexpressionlevelatstageO2maybeduetotheinitiationofformationofprimaryoocytes,andinitialvitellogenesiswithalowlevelofproliferation.Moreover,theexpressionlevelofgraduallyincreasedastheyolkproteinswereaccumulatinginthegrowingoocytesduringvitellogenesis.Amassofproteinsneededtobesynthesizedanddegradedduringgametogenesis,andtheevidenceofubiquitin–conjugatedvitellinpolypeptidesininsectoocyteshadbeenreported.Theseresultsindicatedthatmaybeinvolvedinvitellogenesis.Furthermore,thetestisofcrabcontainsgermcellsofvariousdevelopmentstageswithintheseminiferoustubules.Hence,thehigherexpressionlevelofdevelopmentmaybenecessaryforfastincreasingthenumberofspermatocytecells,whichindicatedthatUPPwasmoreactiveinthesecells.Conclusively,ourstudiesarethefirstreportofcloningandexpressionprofilingofUbincrustaceans.AlloftheseresultscanprovidebasicdatashowingtheroleofSp-Ubwithinthegonaddevelopmentofthecrab.

Abstract:tonguesole()weresub-clonedintotheexpressionvectorspET-32aandpGEX-6P-1,respectively.Subsequently,thesesodiumdodecylsulfate-polyacrylamidegelelectrophoresis(SDS-PAGE).Asaresult,anewspecificGHbandexpressedbyusingthepET-32avector(pET-32a-CSGH)wasfoundwithmolecularmassofabout40kD,andanewspecificGHR-bandexpressedbyusingthepGEX-6P-1vector(pGEX-6P-1-CSGHR1-2)wasfoundwithmolecularmassofabout70kD.TheGHandGHR-recombinantproteinswereexpressedandcomposedof37.6%and20.2%ofthetotalbacterialproteins,respectively.However,pGEX-6P-1-CSGHandtheotherthreerecombinantplasmids(pET-32a-CSGHR1-1,pET-32a-CSGHR1-trans-membranedomainwerenotsuccessfullyexpressed.Therelevantresultsshowedthatthetrans-membranedomainoftheGHR-

Abstract:arebecomingsignificantlyreduced.Fishcultureandartificialhatchingwillhavetoplayagreaterroleinprovidingfloundersources.GermplasmdegradationwithinfishcultureandtheeffectsofartificialhatchesonTherefore,analysisofthegeneticdiversitystatusofthewildfloundergroupattheDNAlevelisveryimportantforprotectionoffloundergermplasmresources.Inthisstudy,inordertoprovidethebasicdatatocorrectlyevaluatethefloundergermplasmresourcesandthegeneticdiversityofthewildgroup,18highlevelpolymorphismmicrosatellitemarkerswereusedtoscreenawildfloundergroupincluding90samples,capturedin2009attheQinhuangdaooffshorearea,HebeiProvince,China.Atotalof161allelesweredetected.Allelenumberinall18locirangedfrom70.86,withanaverageof0.80.ThemeanShannoninformationindexwasestimatedtobe1.9.Theminimumobservedheterozygositywas0.33andthemaximumwas0.64withthemeanbeing0.64.Theminimumexpectedheterozygositywas0.74,themaximumwas0.89andthemeanwas0.84.AtotalofninelociwereprovedsignificantlydeviatedfromtheHardy-WeinbergEquilibriumbytheχ<0.05).TheresultsindicatedthattheoffshoreflounderpopulationwithinQinhuangdaoisavaluablegermplasmresource,richingeneticinformation,andhadanevenalleledistributionandarelativelyhighlevelofgeneticdiversity.Asameasureofprotectingthenaturalgeneticdiversityoftheflounderpopulation,reducingtheimpactofreleasedgroupsonwildgroupsinaneffectivewayandimprovinggeneticdiversityofthefloundergroupintheopensea,brood-stocksinmosthatcheriesconsistedlargelyofculturedfloundersandasmallnumberofwildflounders.Therefore,periodicallychangingspawnbrood-stocksandincreasingthenumberofwildbrood-stockswillbethefirststeptoincreasethegeneticdiversityofreleasedfloundergroups,therebyreducingtheimpactofthehatchfloundersbeingreleasingtotheopenseaonwildfloundergroups.

Abstract:

Abstract:ExperimentswerecarriedouttoinvestigatethebehavioralandmetabolicresponsesoftheNorthPacificseastar)todifferentconcentrationsoffoodsignals(fivesingleaminoacidandthreebivalvetissuehomogenate).Togaingreaterknowledgeintotheforagingbehaviorof,itsbehaviorwasdividedintofourcomponents.Stimulusconcentrationincreaseelicitedariseinboththepercentageofreactinganimalsandinthereactionintensityattheaminoacidconcentration-exceptfortyrosine.Atalowstimulusconcentration,thereactionofconsistedoftubefootwaving,andonlyhighconcentrationsinitiatedacomplicatedsequenceofseveraltypesofbehavior.However,frightreactionswereobservedathighconcentrations(mol·)ofaminoacidexceptforglutamicacid.DifferenceswerefoundamongthebehaviorresponsebyRuditapesphilippinarumantforthestudiedseastars.Fortheeighttestedstimuli,adependencybetweenstimulusconcentrationandtheoxygenconsumptionrate(OCR)wasobserved,withtheincreaseinstimulusstrengthcausingtheincreaseinthemetabolicrate.Forthetissuehomogenatesgroups,theOCRwassignificantlyhigherthanthatinthecontrol.However,statisticallysignificantdifferencesfromthecontrolwereonlyfoundinseastarsexposedtohighconcentrationsofglycine,glutamicacidandasparticacid.Nosignificantdifferenceswereobservedamongdifferentconcentrationsof

Abstract:,healthyshrimpswerevaccinatedwithinactivatedwhitespotsyndromevirus(WSSV).WSSVwasinactivatedusingvaryingdosesofγ-rayirradiation.After20daysofcontinuousfeedingwithinactivatedWSSV,thesurvivingshrimpswereexposedtoactiveWSSVtotesttheirsurvivalrates.Itwasshownthatover216hours,thesurvivalratesattributabletoIE-5kGy,IE-10kGy,IE-15kGyandIE-20kGywere45.5%,54.2%,68%,and65.4%,respectively;themeanWSSVloadsofthesurvivingshrimpswere,comparedwiththatofdeadshrimpsof(3.8±4.4respectively.Withinthepositivecontrolgroup,thesurvivalratewas20%,andthemeanWSSVcopiesofsurvivinganddeadshrimpswere5.8±6.9,respectively.Itissuggestedthatthepresenceofaquasi-immuneresponsesysteminispossible,andexposuretoWSSVinactivatedbyγ-rayirradiationcanimprovetheanti-virusresistanceoftheshrimps.ThesedatashowthatWSSVcanbepreventedbyvaccination,therebyprovidingdatareferencesfordevelopingahealthyculturesystemfor

Abstract:Complementactivationviatheclassical,themannanbindinglectinorthealternativepathwayleadstotheformationoftheterminalcomplementcomplex(TCC).C7playsanimportantroleintheterminalcomplementcascade,sinceC7incorporationintotheTCCallowsitshydrophilic-amphiphilictransition,whichsubsequentlyleadstothedirectbindingofthecomplextotargetcellmembranes.Todate,C7hasbeenclonedonlyfromtheOncorhynchusmykissParalichthysolivaceusHypophthalmichthysmolitrixC7showedthehighestsequenceidentity(82%)withthoseofC7,similartoC7reportedforotherteleostspecies,displayedmanyconservedstructuralmotifs,suchasTSP1,LDLRa,MACPF,EGF,TSP1andCCP.Thismayindicatethattoattacktheexogenousbacteria.WithintheH.molitrixsynonymousmutation,andidentifiedsixalleles.RT-PCRwasconductedtodetecttheexpressionoftheC7geneinvarioustissuesofhealthyAeromonashydrophilaH.molitrix,buttheexpressionofthetargetgeneinthebrain,gill,heart,kidney,liver,intestineandspleenincreasedafter12hofchallengewith.Withtheremovalofpathogens,theexpressiongraduallyreduceduntilacompletedisappearance.ThisresultindicatedthatC7maybean

Abstract:This study assessed the thermal tolerance of 40 turbot families. Growth and upper thermal tolerance traits were recorded. The upper thermal tolerance trait was analyzed using variance analysis, correlation analysis and variance component analysis. Analysis of the two growth stages showed that among the 40 families studied, within the first growth stage, six families showed rapid growth, 26 families showed secondarily rapid growth, and eight families showed common growth. Within the second stage, nine families showed fast growth, 28 families showed secondarily rapid growth, and three families showed common growth. Only one family showed rapid growth over both stages. Analysis of the upper thermal tolerance trait showed that upper thermal tolerance ranged from 836.30–1 367.30℃·h with a total mean of 1 113.00℃·h. Three high heat resistance families were identified. The family with the highest heat resistance was shown to have a mean upper thermal tolerance of 1 367.30℃·h. Five non heat resistance families were identified, with a thermal tolerance of 836.30℃·h identified for the family displaying the lowest mean of upper thermal tolerance. The results of ANOVA showed that there was a significant difference in upper thermal tolerance among the different families studied (P<0.01). The correlation analysis showed a different phenotypic correlation between growth rate at different growth period compared to growth rate and upper thermal tolerance.

Abstract:Temperature-dependentsexdeterminationhasbeendemonstratedinsomespeciesoffishandtemperatureduringtheperiodofsexdifferentiationtypicallyproducesmale-dominantpopulation.ThisresearchinvestigatedthegonadalsexdifferentiationandeffectofrearingtemperatureonthesexratioinlarvalAmursturgeonIn1.5dayspost-hatch,aPGCisvisiblealongthedorsalepitheliumofthebodycavity,betweenthemesenteryandthekidney.Later,inprimordialgermcells(PGC)-PGCsalongthemesentericmigratetobelowthekidneyareaandcellstogethertoformthe60dph,theoriginalgonadaldevelopment,groupsofsmallcellsfromkidneyareamigratetooriginalgonad,-erminalepithelium,fatcellsandbloodvesselsincrease.Putativeovarieswithnotchesinthegerminalepitheliumandpresumedtesteswithsmoothgerminalepitheliumappearedin180Ovarieswithproliferatingoogoniaandearlymeioticoocytesclusterswereobservedin2Inatemperaturecontrolledexperiment,theproportionsoffemaleswere3.3%℃46.7.Theseresultssuggestthatmorphologicalsexdifferentiationin,andnot,butitcanhelptopromotegonadaldevelopmentof

Abstract:andL,mpowderitsindividualatfeedingtimeof12h12hand18hincreasedandwassignificantlyhigherthanotherthreegroups(/Lsuccessively.alsovariedamongdietarytreatmentsofIntermsof∑ω3-PUFApowder∑ω6-PUFA70Thehighestcontentsof50mg/LAt12hand18h,weresignificantlyhigherthanthatofthecontrolgroupighestamountofthesefattyacidswereobservedinAlso,weresignificantlygreatfortreatmentgroupscomparedwithcontrolgrouphadabettereffectforincreasingfattyacidcontentcomparedtootherthreedoses,andthesuitabledurationforincreasefattyacidcontentwas12h.Inconclusion,Antarctickrillcompositioninrotifer

Abstract:onimmunityandantioxidantactivitieswithin).Atotalof210grasscarpindividualsofsimilarinitialweight(45g),wererandomlydividedintotwogroups(threereplicatesforeachgroup).Thecontrolgroupwasfedthebasaldietwhilethetreapreparation.The.Theresultsshowedthattheglobulin,albumintoglobulinratio,immunoglobulinM(IgM)andthecomplement3(C3)levelintheserumofthetreatmentgroupsincreasedby44.62%(P><0.01),respectively,comparedtothecontrol.However,thecontentsoftotalserumprotein,albumin,analyzelysozyme(LZM),myeloperoxidase(MPO)andalkalinephosphatase(AKP)werenotaffectedby>0.05).Comparedwiththecontrol,thetotalantioxidantactivity(T-AOC),glutathioneperoxidase(GSH-Px)andantisuperoxideanionfreeradical(ASAFR)fromliver,serumandthanthoseofcontrol.Themalondialdehyde(MAD)levelintheliversignificantlydecreased,whileintestinalmucosallevelssignificantlyincreasedandtheserumhadnodifferenteffect.Theresultsindicatedthatpreparationsaddedintoculturewatercannotonlypromotetheimmunityandantioxidantabilityofgrasscarp,butalso

Abstract:yellowcatfish(acriticalperiodduringcultivation.Thethetechnicaldifficultyartificiallyhatchedasfoodsourceforlarvalfactory.Thechangesinthegrowth,survival,foodintakeandbodycompositionoftheyellowcatfishlarvaeandjuvenilesfedwithAsthelarvalgrowthanddevelopmentprogressed,thefeedingrateandtherelativesizeofthestomachgraduallyincreased,growingupto10mminsize.Feedingrateswere93.3%,andmostoftheindividualstomachfilledtothe3naupliiexperiencedchangesinproximatenutrientlevels,aminoacidandfattyacidcontentsoverthedifferentgrowthperiods.Amylase,protease,andlipaseactivitiesweredetectableonthefirstdayafterhatching.Duringtheinitialfeedingperiod(day515)thespecificactivitiesofthesethreedigestiveenzymeswererelativelyhigh.Onday15,thespecificactivitiesofthedigestiveenzymesreturnedtoalowlevel.andoodsourceofasuitablesizefor

Abstract:Changesinfishharvestarenotonlyimpactedbyfishing,butalsoinfluencedbyclimatevariability.Basedondataofhairtailcatchesbetween1956and2006intheYellowSeaandBohaiSea,China,theresponsesofcatchestofishingandclimatefactorswereanalyzed.Thecatchtimeseriescanbecompartmentalizedintoaninter-annualtrendandvariations.Theinter-annualtrendwasfishingefforts.Thiswassuggestedbyhighlysignificantregressionofhairtailcatchestofishingeffort.Afterthecatchtrendwasremoved,therewere<0.02)oftheresidualvariationsincatchestowinterwindspeedintheYellowSea,summerwindspeedintheEastChinaSea,andannualprecipitationinYellowRiverValleyandthecoastalregionoftheYellowSeaandBohaiSea,whereastheseasurfacetemperatureandsummerwindspeedintheYellowSea,aswellassummerandwinterwindspeedsintheBohaiSea.Thelinkagesuggeststhattheclimatefactorsgovernthehairtailcatchvariations.ItwasinferredthatrunoffinputnutrientstotheYellowSeaandBohaiSea,whilemonsoonscontrolledthetransportanddistributionofthenutrients,andtheseprocessescanhaveimpactsontheprimaryproductivity.Changesinwatertemperaturecouldalsodirectlyaffectthegrowth,feeding,spawning,andmigrationofthefish,andthus,influencethefishpopulationsize.Thehairtailcatchtimeseriescanbejointlyfittedbyfishingeffortandclimaticfactors(,climatefactorscansignificantlyinfluencethehairtailcatches.Inthecontextofglobalwarming,itwasprojectedthatthefutureclimatechangeswouldleadtoreductioninhairtailproductionintheYellowSeaandBohaiSea,andthehairtailcatchvariationswouldbecomeevengreater.

Abstract:Estuarinesystemsareconsideredasatypicalecotone,andanestuarinefishcommunitystudyisconsideredtobeafundamentalecologicalresearchstudy.Fourfacets(numberofspecies,Pielouevenness,taxonomicdistinctnessandaveragetaxonomicdistinctness)ofcommunitydiversitycharacteristicsstructuringbyspecieslevel,abundance,binarydataandclassificationlevelswereanalyzedwithintheYangtzeRiverestuary,China,whichisdividedintothreezones(Zone1,Zone2,andZone3)alongtheestuarinegradient.Fishsamplingwasconductedusingbottomtrawlingseasonallyfrom20102011.Theresultsshowedthatallfourdiversityparametersdidnotdiffersignificantamongseasons.However,numberofspeciesandaveragetaxonomicdistinctnessbothdifferedsignificantlyamongthedifferentzones.AnincreasingtrendinmeannumbersofspeciesfromZone-1(3.49species)Zone-3(10.13species)wasrelatedtothestrongermarineinfluenceonthelatterzones.LowervaluesofaveragetaxonomicdistinctnessinZone-1suggestedthatmorespecieswerecloselyrelatedinZone-1thaninZone-2orZone-3,forexample,8,20,and19familieswererecordedinZone-1,Zone-2,andZone-3,respectively.Althoughspatialchangesintaxonomicdistinctnessandevennesswerenotsignificant,inversepatternsofthetwoparametersoccurred.Nevertheless,evennesspeakedinZone-1whereasmoreindividualswerecloselyrelatedinZone-1thaninZone-2orZone-3.Moreover,spatialpatternsincommunitydiversitywerestrongerthanseasonalvariations.Thismayreflectthatsalinityplaysthemajorroleindrivingtheestuarinefishcommunity.ThisworkanalyzedthefourfacetsofdiversityestimationsoffishcommunitywithintheYangtzeRiverestuary,andfurtherdemonstratedthatdifferentmeasuredparametersmayhighlightdifferentinformationregardingcommunitystructure,andwillbeusefulforassessingestuarinefishcommunitydiversity.

Abstract:datawereinthefromtinadditiongenerawereidentifiedinDongshanBayandadjacentareas,withPerciformescomprisingthemajororder.Analysisbytheinclusionindexatthetaxonomiclevelshow(genera,species)and(species)perorder,familyandgenusompareDongshanBayandadjacentareasmoredispersed.ThevaluesindicatedthattaxonomicdiversitywithinDongshanBayandadjacentareasPRIMER5.2wasusedtotestt)andthevariationintaxonomicdistinctnessandadjacentareas.Whencomparingwiththoseoftheotherthreeseas,thegeneticrelationshipofthecompositionoffishsmoredistantandhadanunevendistribution.nalyinwinter,springandsummerindexeswerehigherthan1000.Theresultsindicatedthatthedifferentseasons.Tthesedatacanprovidereferencesforsustainabledevelopment,utilizationandconservation

Abstract:Inrecentdecades,thepopulationstructureofmanymarinefishhasdramaticallychangedundertheinfluenceofanthropogenicactivitiesandclimatechange.Tobetterunderstandthechangemechanism,DNAextractedfromformalin-fixedmarinefishspecimensisrequired.However,obtaininghighqualityDNAfromformalin-fixedspecimensisdifficult.Toobtainknowledgeforspecimensthatwerefixedin1962wereusedassamples,andanorthogonalexperimentwasdesignedfollowingtheL)table.TheDNAconcentrationwastakenastheevaluationindex.Thetissuetypes,anti-formaldehydetreatments,digestivetimesandextractionmethodsweretakenasselectionfactors.Thebestgroupwasdeterminedtobe℃anddryinginavacuumcentrifuge,3hofdigestionwithProteinaseKwithinawaterbathof55,andDNAextractionbykit.Anti-formaldehydetreatmentssignificantlyimprovedtheproductionofDNAandtheuseofvariousanti-formaldehydemethodsobtainedthebesteffect.Digestionaftera90DNAwasassessedbyPCRamplificationandsequencingthemethods

Abstract:Brachionusrotundiformisbyextractionsolventsofdifferentmixingproportionwithdifferentextractingtimeswerestudied.Threeextractionsolventsweredesigned(chloroform:methanol(1:2,benzene:petroleumether(1:2,acetone:petroleumether(2:3)toextractfreeze-driedrotiferlipidswith1–3timesextraction.ThelipidsyieldandsaponificationnumbersweredeterminedandwerecomparedwiththeSoxhletextractormethod(nationalstandard).>0.05)affectlipidcontentinalltreatedgroups,andoptimumsolventproportionwasdeterminedtobechloroform:methanol2:1,benzene:petroleumether1:2andacetone:petroleumether2:3.Thelipidandsaponificationnumberofacetone:petroleumether2:3treatmentwere9.71%and198,respectively,andweresignificantly(<0.05)higherthanthatoftheSoxhletextractormethodandothertreatments.Theresultssuggestthatonetimeextractionbyacetone:petroleumether2:3wouldbetheoptimumtreatmentforlipidsextractiononfreeze-driedrotifers.

Abstract:Theuseoffishinggroundanalysisandforecastingmodelcombinedwithgeographicinformationsystemtechnologyintegratingmulti-channeldatafeedsdevelopmentoffisheryreal-timemanagement.Atpresent,thereareseveralproblemsonapplicationofoceanremotesensingonfishinggroundanalysis:irstly,oceanremotesensingwhichcanonlyobtainsurfaceinformationoffishinggroundcombinedwithoceanicverticaldatafromdroguecanexplainfishhabitatconditionsindetail;econdly,fishinggroundcolorelementfromoceanremotesensingisnotaccurateenoughtosupportfishinggroundresourcesresearch;,fishinggroundreal-timeforecastbyremotesensingtechnologyiseasilyinfluencedbyweatherconditions;ourthly,howtobuildscientificevaluationsystemoffishinggroundmodelneedstobesettled;inally,thekeytechnologyofintelligentizedandautomatedfishinggroundanalysisandforecastneedsfurtherresearch.Remotesensingtechnologytothedevelopmentofhighspectralandquantitativedirection,provideimportantconditionsforthequantitativeassessmentofthefisheriesresource.Quantitativeassessmentoftheresourceinventoryofmarinelifeinefficientdevelopmentanduseofmarinebiologicalresources,toguiderationaldevelopmentandsustainableuseofoceanfisheryresourcesisofgreatsignificance.